Transcript: Mouse XM_006508358.3

PREDICTED: Mus musculus synaptotagmin-like 2 (Sytl2), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Sytl2 (83671)
Length:
7510
CDS:
892..6618

Additional Resources:

NCBI RefSeq record:
XM_006508358.3
NBCI Gene record:
Sytl2 (83671)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006508358.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380772 CACAGTCTGGGACCATTATAA pLKO_005 6405 CDS 100% 15.000 21.000 N Sytl2 n/a
2 TRCN0000379670 CAACGAGATATTGCGGTATAA pLKO_005 5901 CDS 100% 13.200 18.480 N Sytl2 n/a
3 TRCN0000381173 AGATCACTCCTTGCAACAAAC pLKO_005 1398 CDS 100% 10.800 15.120 N Sytl2 n/a
4 TRCN0000093351 CCGACCAAGAACCAGATACAA pLKO.1 5390 CDS 100% 5.625 7.875 N Sytl2 n/a
5 TRCN0000093350 GCTAGGAAATTGCCTTCCAAA pLKO.1 5275 CDS 100% 4.950 6.930 N Sytl2 n/a
6 TRCN0000093353 GCAATTCTTAAAGACGCAGAA pLKO.1 5931 CDS 100% 4.050 5.670 N Sytl2 n/a
7 TRCN0000380782 GCACGACTCAAGGGAGTTAAA pLKO_005 6765 3UTR 100% 13.200 10.560 N Sytl2 n/a
8 TRCN0000093352 CCCAGCTCATTAACCAATCTT pLKO.1 5590 CDS 100% 5.625 4.500 N Sytl2 n/a
9 TRCN0000382093 CTCAGAGACCCTCCGTTTAAA pLKO_005 1743 CDS 100% 15.000 10.500 N Sytl2 n/a
10 TRCN0000381381 AGCTCACTAGAGCCGTTAAAG pLKO_005 1864 CDS 100% 13.200 9.240 N Sytl2 n/a
11 TRCN0000379936 AGCTTCCAGTGTGATTGATAT pLKO_005 1296 CDS 100% 13.200 9.240 N Sytl2 n/a
12 TRCN0000381735 GAGTTGGCCTCATGGACAAAT pLKO_005 6675 3UTR 100% 13.200 9.240 N Sytl2 n/a
13 TRCN0000381776 GCGCTCAGATCCGTATGTAAA pLKO_005 5802 CDS 100% 13.200 9.240 N Sytl2 n/a
14 TRCN0000380365 GGAGCAAGACGCCATCTTAAA pLKO_005 921 CDS 100% 13.200 9.240 N Sytl2 n/a
15 TRCN0000380108 GGCTCCAAGAGGGATCCTAAA pLKO_005 1695 CDS 100% 10.800 7.560 N Sytl2 n/a
16 TRCN0000093349 CCGACATTCAAGGAGAGAGTT pLKO.1 6827 3UTR 100% 4.950 3.465 N Sytl2 n/a
17 TRCN0000059949 CGGGATACATTTAAGCGCAAT pLKO.1 5974 CDS 100% 4.050 5.670 N SYTL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006508358.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08416 pDONR223 100% 17.5% 18.7% None (many diffs) n/a
2 ccsbBroad304_08416 pLX_304 0% 17.5% 18.7% V5 (many diffs) n/a
3 TRCN0000467832 ATCTGAGAGGATTAGAATCCCTTC pLX_317 34.8% 17.5% 18.7% V5 (many diffs) n/a
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