Transcript: Mouse XM_006512403.3

PREDICTED: Mus musculus leucine zipper transcription factor-like 1 (Lztfl1), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lztfl1 (93730)
Length:
6660
CDS:
140..988

Additional Resources:

NCBI RefSeq record:
XM_006512403.3
NBCI Gene record:
Lztfl1 (93730)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006512403.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000015139 GCAGCTTATCGAAACATGAAA pLKO.1 893 CDS 100% 5.625 7.875 N LZTFL1 n/a
2 TRCN0000084325 GCCTAAACTTGTTCCAATTAA pLKO.1 493 CDS 100% 15.000 12.000 N Lztfl1 n/a
3 TRCN0000302140 GCCTAAACTTGTTCCAATTAA pLKO_005 493 CDS 100% 15.000 12.000 N Lztfl1 n/a
4 TRCN0000374342 CTTGTACAGTCCCTAAGTATA pLKO_005 1344 3UTR 100% 13.200 10.560 N Lztfl1 n/a
5 TRCN0000233995 CGTATACTTTCACAGTTTAAA pLKO_005 1644 3UTR 100% 15.000 10.500 N Lztfl1 n/a
6 TRCN0000233994 CGTCATGAATGCTGCTTATTA pLKO_005 1556 3UTR 100% 15.000 10.500 N Lztfl1 n/a
7 TRCN0000233996 GGCAATCTTAGGATGATAAAT pLKO_005 2258 3UTR 100% 15.000 10.500 N Lztfl1 n/a
8 TRCN0000374281 AGCTGAGAAGTGGTATCTAAA pLKO_005 358 CDS 100% 13.200 9.240 N Lztfl1 n/a
9 TRCN0000084327 CATTGAAATACAGGCTGTAAA pLKO.1 598 CDS 100% 13.200 9.240 N Lztfl1 n/a
10 TRCN0000374280 GAGGTTTCAGAAGTCCTAAAT pLKO_005 245 CDS 100% 13.200 9.240 N Lztfl1 n/a
11 TRCN0000084326 GCAACTCTTAGGAGTGAATTT pLKO.1 734 CDS 100% 13.200 9.240 N Lztfl1 n/a
12 TRCN0000379023 TGGAGTCCGAGCTCATCAATA pLKO_005 294 CDS 100% 13.200 9.240 N Lztfl1 n/a
13 TRCN0000374343 TTTCCACTCTAGTCAACATTT pLKO_005 1474 3UTR 100% 13.200 9.240 N Lztfl1 n/a
14 TRCN0000084323 CCATGCAAGTACAGTGAGATT pLKO.1 1226 3UTR 100% 4.950 3.465 N Lztfl1 n/a
15 TRCN0000084324 CCATTGAAATACAGGCTGTAA pLKO.1 597 CDS 100% 4.950 3.465 N Lztfl1 n/a
16 TRCN0000302214 CCATTGAAATACAGGCTGTAA pLKO_005 597 CDS 100% 4.950 3.465 N Lztfl1 n/a
17 TRCN0000218129 CATTTAACCGTCACGTGTTAA pLKO_005 1977 3UTR 100% 1.320 0.924 N Lztfl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006512403.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03440 pDONR223 100% 81.6% 85.2% None (many diffs) n/a
2 ccsbBroad304_03440 pLX_304 0% 81.6% 85.2% V5 (many diffs) n/a
3 TRCN0000465884 CCGAACCATAATTAGGCGGTCATG pLX_317 45.4% 81.6% 85.2% V5 (many diffs) n/a
4 ccsbBroadEn_08395 pDONR223 100% 81.2% 84.2% None (many diffs) n/a
5 ccsbBroad304_08395 pLX_304 0% 81.2% 84.2% V5 (many diffs) n/a
6 TRCN0000469753 GGATATGCGTCTAGCGCAAAACCG pLX_317 50.1% 81.2% 84.2% V5 (many diffs) n/a
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