Transcript: Mouse XM_006513482.2

PREDICTED: Mus musculus cell division cycle 34 (Cdc34), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cdc34 (216150)
Length:
1123
CDS:
212..784

Additional Resources:

NCBI RefSeq record:
XM_006513482.2
NBCI Gene record:
Cdc34 (216150)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513482.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422566 GGACGTGTGCATCTCCATTCT pLKO_005 481 CDS 100% 4.950 6.930 N Cdc34 n/a
2 TRCN0000098520 CCATGATCCTTAGGTCCTCTT pLKO.1 885 3UTR 100% 4.050 5.670 N Cdc34 n/a
3 TRCN0000437507 TGACCCACGTCCCTGAAATAA pLKO_005 782 CDS 100% 15.000 10.500 N Cdc34 n/a
4 TRCN0000098524 CTCTTCTACGACGACTACTAT pLKO.1 689 CDS 100% 5.625 3.938 N Cdc34 n/a
5 TRCN0000437524 CCACTCAGCTTTCAGATGATG pLKO_005 969 3UTR 100% 4.950 3.465 N Cdc34 n/a
6 TRCN0000419064 ATGGTGTGAAGGTGCCCACTA pLKO_005 612 CDS 100% 4.050 2.835 N Cdc34 n/a
7 TRCN0000434287 GAGTACTGCGTGAAGACCAAG pLKO_005 641 CDS 100% 4.050 2.835 N Cdc34 n/a
8 TRCN0000434317 GGGATGAAGAGGATGACTCTG pLKO_005 747 CDS 100% 4.050 2.430 N Cdc34 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513482.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00274 pDONR223 100% 74% 80% None (many diffs) n/a
2 ccsbBroad304_00274 pLX_304 0% 74% 80% V5 (many diffs) n/a
3 TRCN0000480218 TCGAACTCATATTTCAATCACCTA pLX_317 54.8% 74% 80% V5 (many diffs) n/a
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