Transcript: Mouse XM_006513585.2

PREDICTED: Mus musculus transducin-like enhancer of split 2 (Tle2), transcript variant X17, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tle2 (21886)
Length:
2927
CDS:
486..2786

Additional Resources:

NCBI RefSeq record:
XM_006513585.2
NBCI Gene record:
Tle2 (21886)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006513585.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000098268 CTGCATCGACATATCAGACTA pLKO.1 2333 CDS 100% 4.950 6.930 N Tle2 n/a
2 TRCN0000288290 CTGCATCGACATATCAGACTA pLKO_005 2333 CDS 100% 4.950 6.930 N Tle2 n/a
3 TRCN0000098267 CTCAACATTGAGATGCATAAA pLKO.1 762 CDS 100% 13.200 10.560 N Tle2 n/a
4 TRCN0000098266 CCTCAAGCTAGAATGTGAGAA pLKO.1 608 CDS 100% 4.950 3.960 N Tle2 n/a
5 TRCN0000295583 GAAGACAAGAGTGACTATAAC pLKO_005 1248 CDS 100% 13.200 9.240 N Tle2 n/a
6 TRCN0000295625 TCAGCAGCTCTGTCGTGTATG pLKO_005 1699 CDS 100% 10.800 7.560 N Tle2 n/a
7 TRCN0000098269 GAAATCTGTGACCGGATCAAA pLKO.1 552 CDS 100% 5.625 3.938 N Tle2 n/a
8 TRCN0000288228 GAAATCTGTGACCGGATCAAA pLKO_005 552 CDS 100% 5.625 3.938 N Tle2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006513585.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01677 pDONR223 100% 81.8% 88.5% None (many diffs) n/a
2 ccsbBroad304_01677 pLX_304 0% 81.8% 88.5% V5 (many diffs) n/a
3 TRCN0000474959 AATGTACAAAGTATTTTTGATATG pLX_317 12.1% 81.8% 88.5% V5 (many diffs) n/a
Download CSV