Construct: ORF TRCN0000474959

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008794.1_s317c1
Derived from:
ccsbBroadEn_01677
DNA Barcode:
AATGTACAAAGTATTTTTGATATG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TLE2 (7089)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474959
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7089 TLE2 TLE family member 2, transc... NM_003260.5 100% 100%
2 human 7089 TLE2 TLE family member 2, transc... NM_001300846.2 99.8% 99.8% 369_371delGCA
3 human 7089 TLE2 TLE family member 2, transc... XM_011528230.1 98.2% 98.2% 369_371delGCA;1139_1140ins36
4 human 7089 TLE2 TLE family member 2, transc... XM_006722864.2 93% 91.8% 369_371delGCA;2049_2050ins77;2079_2080ins76
5 human 7089 TLE2 TLE family member 2, transc... NM_001144761.1 90.7% 89.2% (many diffs)
6 human 7089 TLE2 TLE family member 2, transc... NM_001144762.1 83.5% 83.5% 0_1ins165;202_203ins201
7 mouse 21886 Tle2 transducin-like enhancer of... XM_011243436.2 84.4% 91.4% (many diffs)
8 mouse 21886 Tle2 transducin-like enhancer of... XM_006513589.3 84.3% 91.2% (many diffs)
9 mouse 21886 Tle2 transducin-like enhancer of... XM_011243437.1 84.2% 91.1% (many diffs)
10 mouse 21886 Tle2 transducin-like enhancer of... NM_001252401.1 83.4% 90% (many diffs)
11 mouse 21886 Tle2 transducin-like enhancer of... XM_006513585.2 81.8% 88.5% (many diffs)
12 mouse 21886 Tle2 transducin-like enhancer of... NM_019725.2 81.7% 88.4% (many diffs)
13 mouse 21886 Tle2 transducin-like enhancer of... XM_006513584.2 81.7% 88.4% (many diffs)
14 mouse 21886 Tle2 transducin-like enhancer of... XM_017313922.1 80.4% 87.1% (many diffs)
15 mouse 21886 Tle2 transducin-like enhancer of... XM_006513586.2 76.3% 80.8% (many diffs)
16 mouse 21886 Tle2 transducin-like enhancer of... XM_017313920.1 75.3% 77.9% (many diffs)
17 mouse 21886 Tle2 transducin-like enhancer of... XM_017313919.1 75.2% 77.8% (many diffs)
18 mouse 21886 Tle2 transducin-like enhancer of... XM_011243432.2 75.1% 77.7% (many diffs)
19 mouse 21886 Tle2 transducin-like enhancer of... XM_011243434.2 75.1% 77.7% (many diffs)
20 mouse 21886 Tle2 transducin-like enhancer of... XM_017313921.1 74% 76.5% (many diffs)
21 mouse 21886 Tle2 transducin-like enhancer of... XM_011243433.2 73.9% 76.4% (many diffs)
22 mouse 21886 Tle2 transducin-like enhancer of... XM_011243425.2 73.2% 75.7% (many diffs)
23 mouse 21886 Tle2 transducin-like enhancer of... XM_011243426.2 73.2% 75.7% (many diffs)
24 mouse 21886 Tle2 transducin-like enhancer of... XM_011243435.2 73.1% 75.5% (many diffs)
25 mouse 21886 Tle2 transducin-like enhancer of... XM_011243424.2 73.1% 75.6% (many diffs)
26 mouse 21886 Tle2 transducin-like enhancer of... XM_011243429.2 73.1% 75.6% (many diffs)
27 mouse 21886 Tle2 transducin-like enhancer of... XM_011243427.2 72.1% 74.4% (many diffs)
28 mouse 21886 Tle2 transducin-like enhancer of... XM_011243428.2 71.9% 74.4% (many diffs)
29 mouse 21886 Tle2 transducin-like enhancer of... XM_011243431.2 71.2% 73.6% (many diffs)
30 mouse 21886 Tle2 transducin-like enhancer of... XM_011243430.2 71.2% 73.5% (many diffs)
31 mouse 21886 Tle2 transducin-like enhancer of... XM_017313923.1 70.3% 74.4% (many diffs)
32 mouse 21886 Tle2 transducin-like enhancer of... XM_017313924.1 55.3% 57.6% (many diffs)
33 mouse 21886 Tle2 transducin-like enhancer of... XM_006513588.3 55.2% 57.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2295
ORF length:
2229
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgta cccccaggga aggcacccga ccccgctcca gtccggccag cccttcaagt
121tctcgatctt ggagatctgc gaccgcatca aagaagaatt ccagtttctt caggctcaat
181accacagcct caagctagaa tgtgagaagc tggccagcga gaagacggaa atgcagcgac
241attatgtcat gtattatgag atgtcgtacg ggctcaacat tgaaatgcat aagcaggcgg
301agattgtgaa gcgtctgagc ggtatctgcg ctcagattat ccccttcctg acccaggagc
361atcagcagca ggtgctccag gccgtagaac gcgccaagca ggtcaccgtg ggggagctga
421acagcctcat cgggcagcag ctccagccgc tgtcccacca cgcaccccct gtgcccctca
481ccccccgccc agccgggctg gtgggcggca gtgctacggg gctgcttgct ctgtctggag
541ccctggctgc ccaggctcag ctggcggcgg ctgtcaagga ggaccgtgcg ggcgtggagg
601ccgaggggtc cagagtggag agagccccga gcaggagtgc atctccctcg ccccctgaga
661gtctcgtgga ggaggagcga ccgagtggcc ctggtggtgg cgggaagcag agagcagatg
721agaaggagcc atcaggacct tatgaaagcg acgaagacaa gagtgattac aatctggtgg
781tggacgagga ccaaccctca gagcccccca gcccggctac caccccctgc ggaaaggtac
841ccatctgcat tcctgcccgt cgggacctgg tggacagtcc agcctccttg gcctctagcc
901ttggctcacc gctgcctaga gccaaggagc tcatcctgaa tgaccttccc gccagcactc
961ctgcctccaa atcctgtgac tcctccccgc cccaggacgc ttccaccccc gggcccagct
1021cggccagtca cctctgccag cttgctgcca agccagcacc ttccacggac agcgtcgccc
1081tgaggagccc cctgactctg tccagtccct tcaccacgtc cttcagcctg ggctcccaca
1141gcactctcaa cggagacctc tccgtgccca gctcctacgt cagcctccac ctgtcccccc
1201aggtcagcag ctctgtggtg tacggacgct cccccgtgat ggcatttgag tctcatcccc
1261atctccgagg gtcatccgtc tcttcctccc tacccagcat ccctggggga aagccggcct
1321actccttcca cgtgtctgcg gacgggcaga tgcagccggt tcccttcccc tcggatgcac
1381tggtaggcgc gggcatcccg cggcacgccc ggcagctgca cacgctggcc catggcgagg
1441tggtctgcgc ggtcaccatc agcggctcca cacagcatgt gtacacgggc ggcaagggct
1501gtgtgaaggt gtgggacgtg ggccagcctg gggccaagac gcccgtggcc cagctcgact
1561gcctgaaccg agacaactac attcgttcct gcaagttgct gccggatggc cggagtctga
1621tcgtgggcgg tgaggccagc accttgtcca tttgggacct ggcggcgccc accccccgta
1681tcaaggccga gctgacttcc tcagccccag cctgctacgc cctggccgtc agccccgacg
1741ccaaggtttg cttctcctgc tgcagcgatg gcaacattgt ggtctgggac ctgcagaatc
1801agactatggt caggcagttc cagggccaca cggacggcgc cagctgcatt gatatttccg
1861attacggcac tcggctctgg acagggggcc tggacaacac ggtgcgctgc tgggacctgc
1921gggagggccg ccagctgcag cagcatgact tcagctccca gattttctcc ctgggccact
1981gccctaacca ggactggctg gcggtcggaa tggagagtag caacgtggag atCCTGCACG
2041TCCGCAAGCC GGAGAAATAC CAGCTGCACC TCCACGAGAG CTGCGTGCTG TCCCTGAAGT
2101TTGCCTCCTG CGGACGGTGG TTTGTGAGCA CCGGGAAGGA CAACCTGCTC AACGCCTGGA
2161GGACGCCGTA CGGGGCCAGC ATTTTCCAGT CCAAGGAGTC GTCCTCAGTC CTGAGTTGTG
2221ACATCTCCAG AAATAACAAA TACATCGTGA CAGGCTCGGG GGACAAGAAG GCCACCGTGT
2281ATGAGGTGGT CTACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2341CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2401TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATGTACAAA GTATTTTTGA
2461TATGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)