Transcript: Mouse XM_006514680.3

PREDICTED: Mus musculus IKAROS family zinc finger 1 (Ikzf1), transcript variant X16, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ikzf1 (22778)
Length:
4148
CDS:
172..1617

Additional Resources:

NCBI RefSeq record:
XM_006514680.3
NBCI Gene record:
Ikzf1 (22778)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006514680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430029 AGCTCTTTAGAGGAGCATAAA pLKO_005 709 CDS 100% 13.200 18.480 N Ikzf1 n/a
2 TRCN0000085516 CCGTTGGTAAGCCTCACAAAT pLKO.1 656 CDS 100% 13.200 18.480 N Ikzf1 n/a
3 TRCN0000420145 TTGCCCTAAGCAGAGTTTATG pLKO_005 1973 3UTR 100% 13.200 18.480 N Ikzf1 n/a
4 TRCN0000107874 CGCCAAACGTAAGAGCTCTAT pLKO.1 879 CDS 100% 4.950 6.930 N IKZF1 n/a
5 TRCN0000085514 CGGCCTTATCTACCTAACCAA pLKO.1 1284 CDS 100% 3.000 4.200 N Ikzf1 n/a
6 TRCN0000085517 GCCCTATGACAGTGCCAACTA pLKO.1 939 CDS 100% 4.950 3.960 N Ikzf1 n/a
7 TRCN0000413292 GAGCCAGAGGACCAGATATAA pLKO_005 2067 3UTR 100% 15.000 10.500 N Ikzf1 n/a
8 TRCN0000415420 TATTGTGGCCGGAGCTATAAA pLKO_005 682 CDS 100% 15.000 10.500 N Ikzf1 n/a
9 TRCN0000085513 AGCACAAAGATAGCTGGTTAT pLKO.1 1638 3UTR 100% 10.800 7.560 N Ikzf1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006514680.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11482 pDONR223 100% 74.9% 76.6% None (many diffs) n/a
2 ccsbBroad304_11482 pLX_304 0% 74.9% 76.6% V5 (many diffs) n/a
3 TRCN0000479864 CTGCACAGGTGAAGTGAGACAATT pLX_317 16.8% 74.9% 76.6% V5 (many diffs) n/a
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