Transcript: Mouse XM_006514867.1

PREDICTED: Mus musculus pseudouridylate synthase 10 (Pus10), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pus10 (74467)
Length:
3621
CDS:
485..2104

Additional Resources:

NCBI RefSeq record:
XM_006514867.1
NBCI Gene record:
Pus10 (74467)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006514867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000216556 GATCTATTTGTACGCATATTT pLKO.1 2256 3UTR 100% 15.000 12.000 N Pus10 n/a
2 TRCN0000241410 GATCTATTTGTACGCATATTT pLKO_005 2256 3UTR 100% 15.000 12.000 N Pus10 n/a
3 TRCN0000241408 TTGTCGCTGGCAGATATAATA pLKO_005 1344 CDS 100% 15.000 10.500 N Pus10 n/a
4 TRCN0000241406 CCTGTCCAAGATGCATCTTAA pLKO_005 543 CDS 100% 13.200 9.240 N Pus10 n/a
5 TRCN0000190123 CGAGTGCATTTCACCTCACAA pLKO.1 1571 CDS 100% 4.950 3.465 N Pus10 n/a
6 TRCN0000202403 GAGGGAGTCTCTGTTACTGAA pLKO.1 743 CDS 100% 4.950 3.465 N Pus10 n/a
7 TRCN0000201983 CCTGGACGACTTAAAGGACTT pLKO.1 1771 CDS 100% 4.050 2.835 N Pus10 n/a
8 TRCN0000241407 TTGGGTTCCTGGACGACTTAA pLKO_005 1764 CDS 100% 13.200 7.920 N Pus10 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006514867.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09675 pDONR223 100% 84% 52.3% None (many diffs) n/a
2 ccsbBroad304_09675 pLX_304 0% 84% 52.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000476558 TTTCTCGCGACCAGTAGCAGATCA pLX_317 21.7% 84% 52.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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