Transcript: Mouse XM_006519512.1

PREDICTED: Mus musculus transmembrane and tetratricopeptide repeat containing 4 (Tmtc4), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Tmtc4 (70551)
Length:
3530
CDS:
281..2506

Additional Resources:

NCBI RefSeq record:
XM_006519512.1
NBCI Gene record:
Tmtc4 (70551)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519512.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000198290 GCTAAGTTAGTAGTGGGATTT pLKO.1 341 CDS 100% 10.800 15.120 N Tmtc4 n/a
2 TRCN0000151358 GCTAAGGTTCACTACAACATT pLKO.1 1724 CDS 100% 5.625 7.875 N TMTC4 n/a
3 TRCN0000216948 CGTGGAACAACATGATCATAC pLKO.1 2139 CDS 100% 10.800 7.560 N Tmtc4 n/a
4 TRCN0000151438 GCTTTATTCCTCAAGGCAATT pLKO.1 2297 CDS 100% 10.800 7.560 N TMTC4 n/a
5 TRCN0000178142 GCAACAGTGTAACTTGAAGTT pLKO.1 2955 3UTR 100% 4.950 3.465 N Tmtc4 n/a
6 TRCN0000293092 GCAACAGTGTAACTTGAAGTT pLKO_005 2955 3UTR 100% 4.950 3.465 N Tmtc4 n/a
7 TRCN0000198394 GCAGGATAATATTCCACTGTT pLKO.1 3145 3UTR 100% 4.950 3.465 N Tmtc4 n/a
8 TRCN0000198966 GCGATTAAACACAGGAGGAAA pLKO.1 2006 CDS 100% 4.950 3.465 N Tmtc4 n/a
9 TRCN0000177772 CCTTATGTTCTCATTGGCAAA pLKO.1 2239 CDS 100% 4.050 2.835 N Tmtc4 n/a
10 TRCN0000293093 CCTTATGTTCTCATTGGCAAA pLKO_005 2239 CDS 100% 4.050 2.835 N Tmtc4 n/a
11 TRCN0000182467 GCCATGAATAACCTCGGGAAT pLKO.1 1832 CDS 100% 4.050 2.835 N Tmtc4 n/a
12 TRCN0000293094 GCCATGAATAACCTCGGGAAT pLKO_005 1832 CDS 100% 4.050 2.835 N Tmtc4 n/a
13 TRCN0000178703 CCTGACTTTCAGGATTAACTA pLKO.1 544 CDS 100% 5.625 3.375 N Tmtc4 n/a
14 TRCN0000293095 CCTGACTTTCAGGATTAACTA pLKO_005 544 CDS 100% 5.625 3.375 N Tmtc4 n/a
15 TRCN0000151284 CTGAGAAGAAAGCTAGAACTA pLKO.1 2465 CDS 100% 4.950 3.465 N TMTC4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519512.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14318 pDONR223 100% 45.6% 44.8% None (many diffs) n/a
2 ccsbBroad304_14318 pLX_304 0% 45.6% 44.8% V5 (many diffs) n/a
3 TRCN0000478510 TTTTGAGCACGAAAAGTAATTTTG pLX_317 25.8% 45.6% 44.8% V5 (many diffs) n/a
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