Transcript: Mouse XM_006519576.3

PREDICTED: Mus musculus Rho guanine nucleotide exchange factor (GEF) 3 (Arhgef3), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Arhgef3 (71704)
Length:
3824
CDS:
268..1887

Additional Resources:

NCBI RefSeq record:
XM_006519576.3
NBCI Gene record:
Arhgef3 (71704)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006519576.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000110049 GCTGTTTAGAATCACCCTTCA pLKO.1 1028 CDS 100% 4.050 5.670 N Arhgef3 n/a
2 TRCN0000351733 GCTGTTTAGAATCACCCTTCA pLKO_005 1028 CDS 100% 4.050 5.670 N Arhgef3 n/a
3 TRCN0000110045 CCAGTTTCATTAGCTCTCATT pLKO.1 3068 3UTR 100% 4.950 3.465 N Arhgef3 n/a
4 TRCN0000351735 CCAGTTTCATTAGCTCTCATT pLKO_005 3068 3UTR 100% 4.950 3.465 N Arhgef3 n/a
5 TRCN0000110048 GCGATCTTTGAACTTTCTCAA pLKO.1 685 CDS 100% 4.950 3.465 N Arhgef3 n/a
6 TRCN0000351813 GCGATCTTTGAACTTTCTCAA pLKO_005 685 CDS 100% 4.950 3.465 N Arhgef3 n/a
7 TRCN0000110047 GCGCTGTTTAGAATCACCCTT pLKO.1 1026 CDS 100% 2.640 1.848 N Arhgef3 n/a
8 TRCN0000110046 CCAGGGAATTGTGGCAGAAAT pLKO.1 1191 CDS 100% 13.200 7.920 N Arhgef3 n/a
9 TRCN0000351815 CCAGGGAATTGTGGCAGAAAT pLKO_005 1191 CDS 100% 13.200 7.920 N Arhgef3 n/a
10 TRCN0000047547 CCCATGCTGAAACTCTCCATA pLKO.1 760 CDS 100% 4.950 2.970 N ARHGEF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006519576.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03149 pDONR223 100% 84% 87.9% None (many diffs) n/a
2 ccsbBroad304_03149 pLX_304 0% 84% 87.9% V5 (many diffs) n/a
3 TRCN0000476018 ACGGCTAGCTGTTATCGGGTACTC pLX_317 24.4% 84% 87.9% V5 (many diffs) n/a
4 ccsbBroadEn_11925 pDONR223 100% 52.3% 55.9% None (many diffs) n/a
5 ccsbBroad304_11925 pLX_304 0% 52.3% 55.9% V5 (many diffs) n/a
6 TRCN0000474076 TTTTGCCACTACTCGGCCACGTTG pLX_317 39.2% 52.3% 55.9% V5 (many diffs) n/a
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