Construct: ORF TRCN0000476018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012261.1_s317c1
- Derived from:
- ccsbBroadEn_03149
- DNA Barcode:
- ACGGCTAGCTGTTATCGGGTACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGEF3 (50650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | NM_019555.3 | 100% | 100% | |
| 2 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_011533765.1 | 95.7% | 91.9% | (many diffs) |
| 3 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_011533766.2 | 95.7% | 91.9% | (many diffs) |
| 4 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | NM_001128616.2 | 94.4% | 92.1% | (many diffs) |
| 5 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | NM_001289698.2 | 94.2% | 93.9% | (many diffs) |
| 6 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_017006502.1 | 93.9% | 91.4% | (many diffs) |
| 7 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_024453546.1 | 93.9% | 91.4% | (many diffs) |
| 8 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_024453547.1 | 93.9% | 91.4% | (many diffs) |
| 9 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_011533764.1 | 92.8% | 90.4% | (many diffs) |
| 10 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | NM_001128615.2 | 90.6% | 88.2% | (many diffs) |
| 11 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_005265186.5 | 90.6% | 88.2% | (many diffs) |
| 12 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_005265187.3 | 90.6% | 88.2% | (many diffs) |
| 13 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_017006505.1 | 85.7% | 76.2% | 1174_1175ins49;1353_1354ins176 |
| 14 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_017006506.2 | 80.5% | 68.9% | (many diffs) |
| 15 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_017006504.1 | 80.2% | 68.6% | (many diffs) |
| 16 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_011533767.1 | 78.3% | 78.3% | 0_1ins342 |
| 17 | human | 50650 | ARHGEF3 | Rho guanine nucleotide exch... | XM_017006503.1 | 77.4% | 66.1% | (many diffs) |
| 18 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | NM_001289687.1 | 87.8% | 94.2% | (many diffs) |
| 19 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | XM_006519576.3 | 84% | 87.9% | (many diffs) |
| 20 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | NM_027871.2 | 83.7% | 89.4% | (many diffs) |
| 21 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | NM_001289688.1 | 83.4% | 88.6% | (many diffs) |
| 22 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | NM_001289686.1 | 82% | 86% | (many diffs) |
| 23 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | XM_006519574.3 | 82% | 86% | (many diffs) |
| 24 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | XM_006519575.3 | 82% | 86% | (many diffs) |
| 25 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | XM_006519579.3 | 82% | 86% | (many diffs) |
| 26 | mouse | 71704 | Arhgef3 | Rho guanine nucleotide exch... | XM_006519580.3 | 62.8% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1647
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggccaag gattacccct tctacctcac ggtcaagaga gcgaactgca 121 gcctggagct acccccggcc agcggtccgg ccaaggacgc tgaggagcct agtaataaac 181 gggtcaaacc cctttcccga gtcacgtcgc tagcaaacct catcccgccc gtgaaggcca 241 cgccattaaa gcgcttcagt caaaccctgc agcgctccat tagcttccgc agtgagagcc 301 gccctgacat cctcgccccc cgaccctggt ccagaaatgc cgccccctcg agcacgaaac 361 ggagagatag caagctgtgg agtgagacct tcgatgtgtg cgtcaatcag atgcttacat 421 ccaaggaaat caaacgtcag gaggcgatct ttgagctttc ccaaggagaa gaagacttga 481 tagaagactt gaaattagca aaaaaggcct atcatgaccc catgctgaaa ctctccataa 541 tgacagaaca agagttgaat caaatttttg gaacactgga ctctctaatt cctctacatg 601 aagagctcct tagtcagctt cgagatgtta ggaagcctga tggctcgact gaacatgttg 661 gtcccatcct cgtgggctgg ctcccttgcc tcagctccta tgatagctac tgcagcaatc 721 aagtagccgc caaagctctg ctggaccaca aaaagcaaga tcaccgagtc caggatttcc 781 tacagcgatg tttagaatcc ccctttagcc gcaaactaga tctctggaat ttcctcgata 841 ttccaagaag ccgcctggta aaataccctc tgcttctccg agaaatcttg aggcacacac 901 caaatgataa tccagatcag cagcacttgg aagaagctat aaatatcatt cagggaattg 961 tggcagaaat caacaccaag actggtgaat ctgaatgccg ctattataaa gagcggcttc 1021 tttacttgga agaaggccag aaagactccc tgatcgacag ctctcgagtc ttgtgttgtc 1081 atggtgaact gaagaacaat cggggcgtga aactgcatgt tttcctgttc caagaagtgc 1141 ttgtgatcac tcgagccgtc acccacaatg agcagctttg ctaccagctg taccgtcagc 1201 caatccccgt gaaagacctc ctgctggaag acctccagga tggagaagtg aggctgggtg 1261 gctcccTGCG AGGGGCATTC AGCAACAATG AGAGAATTAA AAACTTCTTC AGAGTCAGTT 1321 TCAAAAATGG ATCCCAAAGT CAGACCCACT CGCTACAAGC CAATGACACT TTCAACAAAC 1381 AGCAGTGGCT TAACTGTATT CGTCAAGCCA AAGAAACAGT TTTGTGTGCT GCCGGGCAAG 1441 CTGGGGTGCT TGACTCCGAG GGATCGTTCC TAAATCCCAC CACCGGGAGC AGAGAGCTAC 1501 AGGGAGAAAC AAAACTTGAG CAGATGGACC AATCGGACAG TGAGTCAGAC TGTAGTATGG 1561 ACACGAGTGA GGTCAGCCTC GACTGTGAGC GCATGGAACA GACAGACTCT TCCTGTGGAA 1621 ACAGCAGGCA CGGTGAAAGT AACGTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACGGCTAG 1801 CTGTTATCGG GTACTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt