Construct: ORF TRCN0000476018

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012261.1_s317c1
Derived from:
ccsbBroadEn_03149
DNA Barcode:
ACGGCTAGCTGTTATCGGGTACTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARHGEF3 (50650)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476018
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 50650 ARHGEF3 Rho guanine nucleotide exch... NM_019555.3 100% 100%
2 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_011533765.1 95.7% 91.9% (many diffs)
3 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_011533766.2 95.7% 91.9% (many diffs)
4 human 50650 ARHGEF3 Rho guanine nucleotide exch... NM_001128616.2 94.4% 92.1% (many diffs)
5 human 50650 ARHGEF3 Rho guanine nucleotide exch... NM_001289698.2 94.2% 93.9% (many diffs)
6 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_017006502.1 93.9% 91.4% (many diffs)
7 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_024453546.1 93.9% 91.4% (many diffs)
8 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_024453547.1 93.9% 91.4% (many diffs)
9 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_011533764.1 92.8% 90.4% (many diffs)
10 human 50650 ARHGEF3 Rho guanine nucleotide exch... NM_001128615.2 90.6% 88.2% (many diffs)
11 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_005265186.5 90.6% 88.2% (many diffs)
12 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_005265187.3 90.6% 88.2% (many diffs)
13 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_017006505.1 85.7% 76.2% 1174_1175ins49;1353_1354ins176
14 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_017006506.2 80.5% 68.9% (many diffs)
15 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_017006504.1 80.2% 68.6% (many diffs)
16 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_011533767.1 78.3% 78.3% 0_1ins342
17 human 50650 ARHGEF3 Rho guanine nucleotide exch... XM_017006503.1 77.4% 66.1% (many diffs)
18 mouse 71704 Arhgef3 Rho guanine nucleotide exch... NM_001289687.1 87.8% 94.2% (many diffs)
19 mouse 71704 Arhgef3 Rho guanine nucleotide exch... XM_006519576.3 84% 87.9% (many diffs)
20 mouse 71704 Arhgef3 Rho guanine nucleotide exch... NM_027871.2 83.7% 89.4% (many diffs)
21 mouse 71704 Arhgef3 Rho guanine nucleotide exch... NM_001289688.1 83.4% 88.6% (many diffs)
22 mouse 71704 Arhgef3 Rho guanine nucleotide exch... NM_001289686.1 82% 86% (many diffs)
23 mouse 71704 Arhgef3 Rho guanine nucleotide exch... XM_006519574.3 82% 86% (many diffs)
24 mouse 71704 Arhgef3 Rho guanine nucleotide exch... XM_006519575.3 82% 86% (many diffs)
25 mouse 71704 Arhgef3 Rho guanine nucleotide exch... XM_006519579.3 82% 86% (many diffs)
26 mouse 71704 Arhgef3 Rho guanine nucleotide exch... XM_006519580.3 62.8% 67.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1647
ORF length:
1578
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggtggccaag gattacccct tctacctcac ggtcaagaga gcgaactgca
121gcctggagct acccccggcc agcggtccgg ccaaggacgc tgaggagcct agtaataaac
181gggtcaaacc cctttcccga gtcacgtcgc tagcaaacct catcccgccc gtgaaggcca
241cgccattaaa gcgcttcagt caaaccctgc agcgctccat tagcttccgc agtgagagcc
301gccctgacat cctcgccccc cgaccctggt ccagaaatgc cgccccctcg agcacgaaac
361ggagagatag caagctgtgg agtgagacct tcgatgtgtg cgtcaatcag atgcttacat
421ccaaggaaat caaacgtcag gaggcgatct ttgagctttc ccaaggagaa gaagacttga
481tagaagactt gaaattagca aaaaaggcct atcatgaccc catgctgaaa ctctccataa
541tgacagaaca agagttgaat caaatttttg gaacactgga ctctctaatt cctctacatg
601aagagctcct tagtcagctt cgagatgtta ggaagcctga tggctcgact gaacatgttg
661gtcccatcct cgtgggctgg ctcccttgcc tcagctccta tgatagctac tgcagcaatc
721aagtagccgc caaagctctg ctggaccaca aaaagcaaga tcaccgagtc caggatttcc
781tacagcgatg tttagaatcc ccctttagcc gcaaactaga tctctggaat ttcctcgata
841ttccaagaag ccgcctggta aaataccctc tgcttctccg agaaatcttg aggcacacac
901caaatgataa tccagatcag cagcacttgg aagaagctat aaatatcatt cagggaattg
961tggcagaaat caacaccaag actggtgaat ctgaatgccg ctattataaa gagcggcttc
1021tttacttgga agaaggccag aaagactccc tgatcgacag ctctcgagtc ttgtgttgtc
1081atggtgaact gaagaacaat cggggcgtga aactgcatgt tttcctgttc caagaagtgc
1141ttgtgatcac tcgagccgtc acccacaatg agcagctttg ctaccagctg taccgtcagc
1201caatccccgt gaaagacctc ctgctggaag acctccagga tggagaagtg aggctgggtg
1261gctcccTGCG AGGGGCATTC AGCAACAATG AGAGAATTAA AAACTTCTTC AGAGTCAGTT
1321TCAAAAATGG ATCCCAAAGT CAGACCCACT CGCTACAAGC CAATGACACT TTCAACAAAC
1381AGCAGTGGCT TAACTGTATT CGTCAAGCCA AAGAAACAGT TTTGTGTGCT GCCGGGCAAG
1441CTGGGGTGCT TGACTCCGAG GGATCGTTCC TAAATCCCAC CACCGGGAGC AGAGAGCTAC
1501AGGGAGAAAC AAAACTTGAG CAGATGGACC AATCGGACAG TGAGTCAGAC TGTAGTATGG
1561ACACGAGTGA GGTCAGCCTC GACTGTGAGC GCATGGAACA GACAGACTCT TCCTGTGGAA
1621ACAGCAGGCA CGGTGAAAGT AACGTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
1681GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1741AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACGGCTAG
1801CTGTTATCGG GTACTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1861aagatt

Download FASTA (ORF) (Full)

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