Transcript: Mouse XM_006523300.2

PREDICTED: Mus musculus quaking (Qk), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Qk (19317)
Length:
4549
CDS:
484..1485

Additional Resources:

NCBI RefSeq record:
XM_006523300.2
NBCI Gene record:
Qk (19317)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006523300.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000233374 TAGGTGCGGTGGCTACTAAAG pLKO_005 1391 CDS 100% 10.800 15.120 N QKI n/a
2 TRCN0000102409 GAGCATCTAAATGAAGACTTA pLKO.1 916 CDS 100% 4.950 3.960 N Qk n/a
3 TRCN0000329417 ATGTACAATGACACGTTAAAT pLKO_005 649 CDS 100% 15.000 10.500 N Qk n/a
4 TRCN0000329482 CCTACAGAGACGCCAACATTA pLKO_005 1091 CDS 100% 13.200 9.240 N Qk n/a
5 TRCN0000102406 CCTAGAGGACTTACAGCTAAA pLKO.1 796 CDS 100% 10.800 7.560 N Qk n/a
6 TRCN0000329480 TCAATCCTTGAGTACCCTATT pLKO_005 1354 CDS 100% 10.800 7.560 N Qk n/a
7 TRCN0000015184 CCCTACCATAATGCCTTTGAT pLKO.1 1203 CDS 100% 5.625 3.938 N QKI n/a
8 TRCN0000015185 AGAAACTTTATGTGCCTGTAA pLKO.1 734 CDS 100% 4.950 3.465 N QKI n/a
9 TRCN0000015187 GACGAAGAAATTAGCAGAGTA pLKO.1 619 CDS 100% 4.950 3.465 N QKI n/a
10 TRCN0000102407 GCACCAGCTACATCAATCCTT pLKO.1 1342 CDS 100% 3.000 2.100 N Qk n/a
11 TRCN0000015183 GCTACATCAATCCTTGAGTAT pLKO.1 1348 CDS 100% 4.950 3.465 N QKI n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006523300.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02164 pDONR223 100% 94% 97.6% None (many diffs) n/a
2 ccsbBroad304_02164 pLX_304 0% 94% 97.6% V5 (many diffs) n/a
3 TRCN0000467894 CCTGACTCCGTGTACCCTTCACCC pLX_317 41.9% 94% 97.6% V5 (many diffs) n/a
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