Construct: ORF TRCN0000467894
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002417.1_s317c1
- Derived from:
- ccsbBroadEn_02164
- DNA Barcode:
- CCTGACTCCGTGTACCCTTCACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QKI (9444)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467894
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9444 | QKI | QKI, KH domain containing R... | NM_006775.3 | 100% | 100% | |
| 2 | human | 9444 | QKI | QKI, KH domain containing R... | XM_011536258.2 | 99.7% | 99.7% | 634_635insCAG |
| 3 | human | 9444 | QKI | QKI, KH domain containing R... | XM_017011502.1 | 98.6% | 98.5% | 1010_1011delGTinsCCGCCACCGGCAAC |
| 4 | human | 9444 | QKI | QKI, KH domain containing R... | NM_001301085.2 | 97.6% | 97.6% | 634_635ins24 |
| 5 | human | 9444 | QKI | QKI, KH domain containing R... | NM_206854.3 | 93.1% | 92% | (many diffs) |
| 6 | human | 9444 | QKI | QKI, KH domain containing R... | NM_206853.3 | 93% | 92.1% | (many diffs) |
| 7 | human | 9444 | QKI | QKI, KH domain containing R... | NM_206855.3 | 92.9% | 91.4% | (many diffs) |
| 8 | human | 9444 | QKI | QKI, KH domain containing R... | XM_017011503.1 | 92.7% | 91.8% | (many diffs) |
| 9 | human | 9444 | QKI | QKI, KH domain containing R... | XM_011536259.2 | 90.7% | 89.7% | (many diffs) |
| 10 | human | 9444 | QKI | QKI, KH domain containing R... | XM_017011504.1 | 90.7% | 89.7% | (many diffs) |
| 11 | human | 9444 | QKI | QKI, KH domain containing R... | XM_011536260.2 | 85.9% | 85.9% | 402_403ins144 |
| 12 | human | 9444 | QKI | QKI, KH domain containing R... | XM_011536261.1 | 55.6% | 51.8% | (many diffs) |
| 13 | human | 9444 | QKI | QKI, KH domain containing R... | XR_245557.3 | 12.5% | (many diffs) | |
| 14 | human | 9444 | QKI | QKI, KH domain containing R... | XR_942633.2 | 12.5% | (many diffs) | |
| 15 | human | 9444 | QKI | QKI, KH domain containing R... | XR_001743750.1 | 10.5% | (many diffs) | |
| 16 | human | 9444 | QKI | QKI, KH domain containing R... | XR_001743749.1 | 10.1% | (many diffs) | |
| 17 | mouse | 19317 | Qk | quaking | NM_001159517.1 | 96.3% | 100% | (many diffs) |
| 18 | mouse | 19317 | Qk | quaking | XM_006523300.2 | 94% | 97.6% | (many diffs) |
| 19 | mouse | 19317 | Qk | quaking | NM_021881.2 | 89.5% | 92% | (many diffs) |
| 20 | mouse | 19317 | Qk | quaking | NM_001159516.1 | 89.4% | 92.1% | (many diffs) |
| 21 | mouse | 19317 | Qk | quaking | XR_385168.2 | 17.7% | (many diffs) | |
| 22 | mouse | 19317 | Qk | quaking | XR_385170.2 | 17.2% | (many diffs) | |
| 23 | mouse | 19317 | Qk | quaking | XR_385167.2 | 14.3% | (many diffs) | |
| 24 | mouse | 19317 | Qk | quaking | XR_385169.2 | 13.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt cggggaaatg gaaacgaagg agaagccgaa gcccacccca gattacctga 121 tgcagctgat gaacgacaag aagctcatga gcagcctgcc caacttctgc gggatcttca 181 accacctcga gcggctgctg gacgaagaaa ttagcagagt acggaaagac atgtacaatg 241 acacattaaa tggcagtaca gagaaaagga gtgcagaatt gcctgatgct gtgggaccta 301 ttgttcagtt acaagagaaa ctttatgtgc ctgtaaaaga atacccagat tttaattttg 361 ttgggagaat ccttggacct agaggactta cagccaaaca acttgaagca gaaaccggat 421 gtaaaatcat ggtccgaggc aaaggctcaa tgagggataa aaaaaaggag gagcaaaata 481 gaggcaagcc caattgggag catctaaatg aagatttaca tgtactaatc actgtggaag 541 atgctcagaa cagagcagaa atcaaattga agagagcagt tgaagaagtg aagaaattat 601 tggtacctgc agcagaagga gaagacagcc tgaagaagat gcagctgatg gagcttgcga 661 tTCTGAATGG CACCTACAGA GATGCCAACA TTAAATCACC AGCCCTTGCC TTTTCTCTTG 721 CAGCAACAGC CCAGGCTGCT CCAAGGATCA TTACTGGGCC TGCGCCGGTT CTCCCACCAG 781 CTGCCCTGCG TACTCCTACG CCAGCTGGCC CTACCATAAT GCCTTTGATC AGACAAATAC 841 AGACCGCTGT CATGCCAAAC GGAACTCCTC ACCCAACTGC TGCAATAGTT CCTCCAGGGC 901 CCGAAGCTGG TTTAATCTAT ACACCCTATG AGTACCCCTA CACATTGGCA CCAGCTACAT 961 CAATCCTTGA GTATCCTATT GAACCTAGTG GTGTATTAGG TGCGGTGGCT ACTAAAGTTC 1021 GAAGGCACGA TATGCGTGTC CATCCTTACC AAAGGATTGT GACCGCAGAC CGAGCCGCCA 1081 CCGGCAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCTGAC TCCGTGTACC CTTCACCCAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt