Construct: ORF ccsbBroad304_14921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018857.1_s304c1, BRDN0000459341
- Derived from:
- ccsbBroadEn_14921
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDKL2 (8999)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14921
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_033770.1 | 19.8% | (many diffs) | |
| 2 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_160778.1 | 19.8% | (many diffs) | |
| 3 | human | 727758 | ROCK1P1 | Rho associated coiled-coil ... | NR_160777.1 | 18.2% | (many diffs) | |
| 4 | human | 6093 | ROCK1 | Rho associated coiled-coil ... | NM_005406.3 | 12% | 9.8% | (many diffs) |
| 5 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | XM_006525725.1 | 11% | 9.5% | (many diffs) |
| 6 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | NM_009071.2 | 11% | 9.5% | (many diffs) |
| 7 | mouse | 19877 | Rock1 | Rho-associated coiled-coil ... | XR_001782350.1 | 7.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 507
- ORF length:
- 438
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctatatt tattttcatt ttagtaaact gtttcacgtt agacctgtaa 121 cccaaggaga tgtgtataga gctgaaactg aagaaattcc taaaatattc cagatactat 181 atgcaaatga aggtgaatgt agaaaagatg tagagatgga accagtacaa gaagctgaaa 241 aaactaattt ccaaaatcac aaaggccatg agtttattcc tacactctac cactttcctg 301 ccaattgtga tgcctgtgcc aaacctctct ggcatgtttt taagccaccc cctgccctag 361 agtgtcgaag atgccatgtt aagtgccaca gagatcactt agataagaaa gaggacttaa 421 tttgtccatg taaagtaagt tatgatgtaa catcagcaag agatatgctg ctgttagcat 481 gttctcagga tgaacaaaaa aatgggtaac tcatttagta aagaaaatcc ctaagaatcc 541 accatctggt tttgttcgtg cttccccttt gccaactttc ttgtacaaag tggttggtaa 601 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg 661 gtggcgcgtt aagtcgacaa tcaacctctg gatta