Transcript: Mouse XM_006534345.3

PREDICTED: Mus musculus prostaglandin E synthase 3 (cytosolic)-like (Ptges3l), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ptges3l (73635)
Length:
892
CDS:
85..534

Additional Resources:

NCBI RefSeq record:
XM_006534345.3
NBCI Gene record:
Ptges3l (73635)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006534345.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000115189 GCTCTCTGTGGACTTCGATAA pLKO.1 381 CDS 100% 10.800 8.640 N Ptges3l n/a
2 TRCN0000115188 AGGCTCACAAAGGAGGATATA pLKO.1 349 CDS 100% 13.200 9.240 N Ptges3l n/a
3 TRCN0000115187 GAGCTGTATAACGAGATTGAA pLKO.1 229 CDS 100% 5.625 3.938 N Ptges3l n/a
4 TRCN0000115186 GCCTTCTTCAACTACTTGGTT pLKO.1 608 3UTR 100% 3.000 2.100 N Ptges3l n/a
5 TRCN0000115190 CATTACTTGCTTTGTGAGGAA pLKO.1 303 CDS 100% 2.640 1.848 N Ptges3l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006534345.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09044 pDONR223 100% 21.1% 21.5% None (many diffs) n/a
2 ccsbBroad304_09044 pLX_304 0% 21.1% 21.5% V5 (many diffs) n/a
3 TRCN0000467834 ACAGACACAACCAACTTTGAGGAA pLX_317 28.9% 21.1% 21.5% V5 (many diffs) n/a
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