Construct: ORF TRCN0000467834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013648.1_s317c1
- Derived from:
- ccsbBroadEn_09044
- DNA Barcode:
- ACAGACACAACCAACTTTGAGGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AARSD1 (80755)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100885850 | PTGES3L-AARSD1 | PTGES3L-AARSD1 readthrough | NM_025267.3 | 99.9% | 100% | 399C>T |
| 2 | human | 100885850 | PTGES3L-AARSD1 | PTGES3L-AARSD1 readthrough | NM_001136042.2 | 89.5% | 89.5% | 1_183del;582C>T |
| 3 | human | 80755 | AARSD1 | alanyl-tRNA synthetase doma... | NM_001261434.2 | 77.5% | 76.5% | (many diffs) |
| 4 | human | 100885848 | PTGES3L | prostaglandin E synthase 3 ... | NM_001142654.1 | 22.9% | 18.6% | (many diffs) |
| 5 | human | 100885848 | PTGES3L | prostaglandin E synthase 3 ... | NM_001261430.1 | 22.9% | 21.8% | (many diffs) |
| 6 | mouse | 69684 | Aarsd1 | alanyl-tRNA synthetase doma... | NM_144829.1 | 68.3% | 69.1% | (many diffs) |
| 7 | mouse | 69684 | Aarsd1 | alanyl-tRNA synthetase doma... | XM_006534121.2 | 39.4% | 40.3% | (many diffs) |
| 8 | mouse | 73635 | Ptges3l | prostaglandin E synthase 3 ... | XM_006534346.3 | 21.8% | 22.2% | (many diffs) |
| 9 | mouse | 73635 | Ptges3l | prostaglandin E synthase 3 ... | XM_011249282.2 | 21.8% | 22.2% | (many diffs) |
| 10 | mouse | 73635 | Ptges3l | prostaglandin E synthase 3 ... | XM_006534345.3 | 21.1% | 21.5% | (many diffs) |
| 11 | mouse | 73635 | Ptges3l | prostaglandin E synthase 3 ... | NM_026865.2 | 18% | 18.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttttgtgtt gaggacagca ccgatgtcca cgtgcttatt gaggatcacc 121 gcattgtgtt cagctgcaag aatgccgatg gagtggagtt gtacaatgag attgagttct 181 atgccaaagt gaactccaag gactcccagg ataagcgctc ttcccgctct attacttgtt 241 ttgtgagaaa atggaaggaa aaggtggcct ggccgcggct taccaaggag gatatcaagc 301 cagtgtggct gtctgtggac tttgataact ggagagactg ggaaggggat gaagagatgg 361 agctggctca tgtggaacat tatgcagagc ttttgaagaa ggtcagcacc aagagacctc 421 cacctgccat ggatgatttg gatttcacca ccaccgtggt ctcttgctgt cccgcggagc 481 tgcagactga agggagcaac ggcaagaaag aagtgctgag cggtttccaa gtggtgctgg 541 aagacacagt gcttttccct gagggcgggg gacagcctga tgaccgtggt acaatcaatg 601 acatctctgt gctgagagtg actcgccgtg gggaacaggc tgatcatttc acccagacac 661 ccctggatcc aggaagccag gttctggtcc gggtagattg ggagcggagg tttgaccaca 721 tgcagcagca ttcagggcag catctcatca cggcagttgc tgaccatcta tttaagctga 781 agacaacatc atgggagtta gggagatttc ggagtgcgat tgagctggac accccctcta 841 tgactgcaga gcaagtagct gccattgagc agagcgtcaa tgaaaaaatc agagatcggc 901 tgcctgtgaa tgtccgagaa ctgagcctgg atgatcctga ggtggagcag gtgagtggcc 961 ggggtttgcc tgatgatcat gctgggccca ttcgggttgt taacatcgag ggcgttgatt 1021 ccaacatgtg ctgtgggacc catgtgagca atctcagtga ccttcaggtc attaagattc 1081 tgggcactga gaaggggaaa aagaacagaa ccaacctgat atttctgtct gggaaccggg 1141 tgctgaagtg gatggagaga agtcatggaa ctgaaaaagc actgactgcT CTGCTTAAGT 1201 GTGGAGCAGA GGATCATGTG GAAGCAGTGA AAAAGCTCCA GAACTCCACC AAGATCCTGC 1261 AGAAGAATAA CCTGAATCTG CTCAGAGACC TGGCTGTGCA CATTGCCCAT AGCCTCAGGA 1321 ACAGTCCAGA CTGGGGAGGT GTGGTCATAT TACACAGGAA GGAGGGTGAT TCAGAGTTCA 1381 TGAATATCAT TGCCAATGAG ATTGGGTCAG AGGAGACCCT CCTGTTCTTA ACTGTGGGCG 1441 ATGAGAAAGG TGGTGGACTC TTCTTACTGG CAGGGCCACC TGCGTCTGTG GAGACCCTGG 1501 GGCCCAGGGT GGCTGAGGTC CTGGAAGGCA AAGGAGCAGG GAAGAAAGGC CGTTTTCAGG 1561 GCAAGGCCAC CAAGATGAGC CGGCGGATGG AGGCGCAGGC GCTTCTCCAG GACTACATCA 1621 GCACGCAGAG TGCTAAGGAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1681 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1741 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAG ACACAACCAA 1801 CTTTGAGGAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt