Construct: ORF ccsbBroad304_09044

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013648.1_s304c1, BRDN0000403018
Derived from:
ccsbBroadEn_09044
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AARSD1 (80755)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09044
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 100885850 PTGES3L-AARSD1 PTGES3L-AARSD1 readthrough NM_025267.3 99.9% 100% 399C>T
2 human 100885850 PTGES3L-AARSD1 PTGES3L-AARSD1 readthrough NM_001136042.2 89.5% 89.5% 1_183del;582C>T
3 human 80755 AARSD1 alanyl-tRNA synthetase doma... NM_001261434.2 77.5% 76.5% (many diffs)
4 human 100885848 PTGES3L prostaglandin E synthase 3 ... NM_001142654.1 22.9% 18.6% (many diffs)
5 human 100885848 PTGES3L prostaglandin E synthase 3 ... NM_001261430.1 22.9% 21.8% (many diffs)
6 mouse 69684 Aarsd1 alanyl-tRNA synthetase doma... NM_144829.1 68.3% 69.1% (many diffs)
7 mouse 69684 Aarsd1 alanyl-tRNA synthetase doma... XM_006534121.2 39.4% 40.3% (many diffs)
8 mouse 73635 Ptges3l prostaglandin E synthase 3 ... XM_006534346.3 21.8% 22.2% (many diffs)
9 mouse 73635 Ptges3l prostaglandin E synthase 3 ... XM_011249282.2 21.8% 22.2% (many diffs)
10 mouse 73635 Ptges3l prostaglandin E synthase 3 ... XM_006534345.3 21.1% 21.5% (many diffs)
11 mouse 73635 Ptges3l prostaglandin E synthase 3 ... NM_026865.2 18% 18.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1641
ORF length:
1575
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gttttgtgtt gaggacagca ccgatgtcca cgtgcttatt gaggatcacc
121gcattgtgtt cagctgcaag aatgccgatg gagtggagtt gtacaatgag attgagttct
181atgccaaagt gaactccaag gactcccagg ataagcgctc ttcccgctct attacttgtt
241ttgtgagaaa atggaaggaa aaggtggcct ggccgcggct taccaaggag gatatcaagc
301cagtgtggct gtctgtggac tttgataact ggagagactg ggaaggggat gaagagatgg
361agctggctca tgtggaacat tatgcagagc ttttgaagaa ggtcagcacc aagagacctc
421cacctgccat ggatgatttg gatttcacca ccaccgtggt ctcttgctgt cccgcggagc
481tgcagactga agggagcaac ggcaagaaag aagtgctgag cggtttccaa gtggtgctgg
541aagacacagt gcttttccct gagggcgggg gacagcctga tgaccgtggt acaatcaatg
601acatctctgt gctgagagtg actcgccgtg gggaacaggc tgatcatttc acccagacac
661ccctggatcc aggaagccag gttctggtcc gggtagattg ggagcggagg tttgaccaca
721tgcagcagca ttcagggcag catctcatca cggcagttgc tgaccatcta tttaagctga
781agacaacatc atgggagtta gggagatttc ggagtgcgat tgagctggac accccctcta
841tgactgcaga gcaagtagct gccattgagc agagcgtcaa tgaaaaaatc agagatcggc
901tgcctgtgaa tgtccgagaa ctgagcctgg atgatcctga ggtggagcag gtgagtggcc
961ggggtttgcc tgatgatcat gctgggccca ttcgggttgt taacatcgag ggcgttgatt
1021ccaacatgtg ctgtgggacc catgtgagca atctcagtga ccttcaggtc attaagattc
1081tgggcactga gaaggggaaa aagaacagaa ccaacctgat atttctgtct gggaaccggg
1141tgctgaagtg gatggagaga agtcatggaa ctgaaaaagc actgactgct ctgcttaagt
1201gtggagcaga ggatcatgtg gaagcagtga aaaagctcca gaactccacc aagatcctgc
1261agaagaataa cctgaatctg ctcagagacc tggctgtgca cattgcccat agcctcagga
1321acagtccaga ctggggaggt gtggtcatat tacacaggaa ggagggtgat tcagagttca
1381tgaatatcat tgccaatgag attgggtcag aggagaccct cctgttctta actgtgggcg
1441atgagaaagg tggtggactc ttcttactgg cagggccacc tgcgtctgtg gagaccctgg
1501ggcccagggt ggctgaggtc ctggaaggca aaggagcagg gaagaaaggc cgttttcagg
1561gcaaggccac caagatgagc cggcggatgg aggcgcaggc gcttctccag gactacatca
1621gcacgcagag tgctaaggag tgcccaactt tcttgtacaa agtggttggt aagcctatcc
1681ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1741ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)