Transcript: Mouse XM_006535276.3

PREDICTED: Mus musculus E1A binding protein p400 (Ep400), transcript variant X12, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ep400 (75560)
Length:
11052
CDS:
431..9628

Additional Resources:

NCBI RefSeq record:
XM_006535276.3
NBCI Gene record:
Ep400 (75560)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535276.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305480 GTCGTCAGAAGGCCTTATATG pLKO_005 4416 CDS 100% 13.200 18.480 N Ep400 n/a
2 TRCN0000109315 CCGTGAACATTAGCTTTGATT pLKO.1 9985 3UTR 100% 5.625 7.875 N Ep400 n/a
3 TRCN0000109316 CGGAAGAATCTCAATGGCATA pLKO.1 3746 CDS 100% 4.050 5.670 N Ep400 n/a
4 TRCN0000109319 CCAGATTAAAGGGATTTGATA pLKO.1 3174 CDS 100% 5.625 4.500 N Ep400 n/a
5 TRCN0000109318 CCTCAAGTTGTGCAGCAACAA pLKO.1 9290 CDS 100% 4.950 3.960 N Ep400 n/a
6 TRCN0000351333 CCTCAAGTTGTGCAGCAACAA pLKO_005 9290 CDS 100% 4.950 3.960 N Ep400 n/a
7 TRCN0000305479 ATAGCTCAGCTGGCGTCTATT pLKO_005 5000 CDS 100% 13.200 9.240 N Ep400 n/a
8 TRCN0000311325 TGCTACTTTCCAGTCCATTAA pLKO_005 8152 CDS 100% 13.200 9.240 N Ep400 n/a
9 TRCN0000311323 TACTTGGTAGGACTCCATATC pLKO_005 9807 3UTR 100% 10.800 7.560 N Ep400 n/a
10 TRCN0000109317 GCTGACTTTATGGAACAGCTT pLKO.1 6635 CDS 100% 2.640 1.848 N Ep400 n/a
11 TRCN0000087583 AGGAGGAAGAGGAAGAGGAAA pLKO.1 1716 CDS 100% 4.950 2.475 Y Adam32 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535276.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12379 pDONR223 100% 26.3% 27.4% None (many diffs) n/a
2 ccsbBroad304_12379 pLX_304 17.3% 26.3% 27.4% V5 (many diffs) n/a
3 TRCN0000478600 ACGCTGATGCCGCATAAATTTCTC pLX_317 11.8% 26.3% 27.4% V5 (many diffs) n/a
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