Construct: ORF TRCN0000471852

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018457.1_s317c1
Derived from:
ccsbBroadEn_12508
DNA Barcode:
TGATTCATGTCCCCATCGGCCGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NDRG4 (65009)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471852
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65009 NDRG4 NDRG family member 4 NM_001242836.1 100% 100%
2 human 65009 NDRG4 NDRG family member 4 NM_001242835.1 96.3% 96% 866_904del
3 human 65009 NDRG4 NDRG family member 4 NM_001242834.1 94.9% 94.9% 21_74del
4 human 65009 NDRG4 NDRG family member 4 NM_001242833.1 91.8% 91.8% 21_110del
5 human 65009 NDRG4 NDRG family member 4 XM_006721262.3 91.6% 91.3% 21_74del;920_958del
6 human 65009 NDRG4 NDRG family member 4 NM_020465.3 90.9% 89.5% (many diffs)
7 human 65009 NDRG4 NDRG family member 4 NM_022910.3 90.9% 89.5% (many diffs)
8 human 65009 NDRG4 NDRG family member 4 XM_017023590.1 90.9% 89.5% (many diffs)
9 human 65009 NDRG4 NDRG family member 4 XM_006721261.3 88.7% 88.4% 21_110del;956_994del
10 human 65009 NDRG4 NDRG family member 4 XM_017023589.2 87.8% 86.4% (many diffs)
11 human 65009 NDRG4 NDRG family member 4 XM_006721260.4 87.8% 86.2% (many diffs)
12 human 65009 NDRG4 NDRG family member 4 XM_011523293.2 87.8% 86.2% (many diffs)
13 human 65009 NDRG4 NDRG family member 4 XM_011523294.2 87.8% 86.2% (many diffs)
14 human 65009 NDRG4 NDRG family member 4 XM_017023582.2 86.6% 85.6% (many diffs)
15 human 65009 NDRG4 NDRG family member 4 XM_017023588.1 86.6% 85.6% (many diffs)
16 human 65009 NDRG4 NDRG family member 4 NM_001130487.1 86.2% 84.9% (many diffs)
17 human 65009 NDRG4 NDRG family member 4 XM_006721259.4 84.9% 83.4% (many diffs)
18 human 65009 NDRG4 NDRG family member 4 XM_011523292.3 84.2% 82.7% (many diffs)
19 human 65009 NDRG4 NDRG family member 4 XM_017023587.1 84.2% 82.7% (many diffs)
20 human 65009 NDRG4 NDRG family member 4 XM_017023586.1 83.8% 82.8% (many diffs)
21 human 65009 NDRG4 NDRG family member 4 XM_006721258.3 83.8% 82.5% (many diffs)
22 human 65009 NDRG4 NDRG family member 4 XM_011523291.3 83.8% 82.5% (many diffs)
23 human 65009 NDRG4 NDRG family member 4 NM_001363869.1 83.7% 83.7% 0_1ins165
24 human 65009 NDRG4 NDRG family member 4 XM_006721257.4 83.4% 81.9% (many diffs)
25 human 65009 NDRG4 NDRG family member 4 XM_017023585.1 82.3% 81.4% (many diffs)
26 human 65009 NDRG4 NDRG family member 4 XM_006721256.3 81.6% 79.9% (many diffs)
27 human 65009 NDRG4 NDRG family member 4 XM_011523290.3 81.6% 79.9% (many diffs)
28 human 65009 NDRG4 NDRG family member 4 XM_024450398.1 81.6% 79.9% (many diffs)
29 human 65009 NDRG4 NDRG family member 4 XM_024450400.1 80.6% 80.3% 0_1ins165;701_739del
30 human 65009 NDRG4 NDRG family member 4 XM_017023584.1 80.2% 78.8% (many diffs)
31 human 65009 NDRG4 NDRG family member 4 XM_006721254.3 79.8% 78.6% (many diffs)
32 human 65009 NDRG4 NDRG family member 4 XM_006721253.3 79.1% 77.5% (many diffs)
33 human 65009 NDRG4 NDRG family member 4 XM_006721252.3 77.8% 76.2% (many diffs)
34 human 65009 NDRG4 NDRG family member 4 XM_017023583.2 71.3% 70% (many diffs)
35 human 65009 NDRG4 NDRG family member 4 NR_040072.1 30.9% 1_106del;127_218del;1216_3282del
36 mouse 234593 Ndrg4 N-myc downstream regulated ... NM_145602.3 91.2% 97.9% (many diffs)
37 mouse 234593 Ndrg4 N-myc downstream regulated ... NM_001195006.1 87.8% 94% (many diffs)
38 mouse 234593 Ndrg4 N-myc downstream regulated ... XM_006530869.1 80% 84.4% (many diffs)
39 mouse 234593 Ndrg4 N-myc downstream regulated ... XM_006530868.1 78.5% 83.1% (many diffs)
40 mouse 234593 Ndrg4 N-myc downstream regulated ... XM_006530867.1 76% 80.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1083
ORF length:
1017
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ggagtgctgg gatggggaac atgacatcga gacaccctac ggccttctgc
121atgtagtgat ccggggctcc cccaagggga accgcccagc catcctcacc taccatgatg
181tgggcctcaa ccacaaacta tgcttcaaca ccttcttcaa cttcgaggac atgcaggaga
241tcaccaagca ctttgtggtg tgtcacgtgg atgcccctgg acaacaggtg ggggcgtcgc
301agtttcctca ggggtaccag ttcccctcca tggagcagct ggctgccatg ctccccagcg
361tggtgcagca tttcgggttc aagtatgtga ttggcatcgg agtgggcgcc ggagcctatg
421tgctggccaa gtttgcactc atcttccccg acctggtgga ggggctggtg ctggtgaaca
481tcgaccccaa tggcaaaggc tggatagact gggctgccac caagctctcc ggcctaacta
541gcactttacc cgacacggtg ctctcccacc TCTTCAGCCA GGAGGAGCTG GTGAACAACA
601CAGAGTTGGT GCAGAGCTAC CGGCAGCAGA TTGGGAACGT GGTGAACCAG GCCAACCTGC
661AGCTCTTCTG GAACATGTAC AACAGCCGCA GAGACCTGGA CATTAACCGG CCTGGAACGG
721TGCCCAATGC CAAGACGCTC CGCTGCCCCG TGATGCTGGT GGTTGGGGAT AATGCACCCG
781CTGAGGACGG GGTGGTGGAG TGCAACTCCA AACTGGACCC GACCACTACG ACCTTCCTGA
841AGATGGCAGA CTCTGGAGGG CTGCCCCAGG TCACACAGCC AGGGAAGCTG ACTGAAGCCT
901TCAAATACTT CCTGCAAGGC ATGGGCTACA TGCCCTCAGC CAGCATGACC CGCCTGGCAC
961GCTCCCGCAC TGCATCCCTC ACCAGTGCCA GCTCGGTGGA TGGCAGCCGC CCACAGGCCT
1021GCACCCACTC AGAGAGCAGC GAGGGGCTGG GCCAGGTCAA CCACACCATG GAGGTGTCCT
1081GTTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1141TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1201GGCTTTATAT ATCTTGTGGA AAGGACGATG ATTCATGTCC CCATCGGCCG GGACGCGTTA
1261AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)