Transcript: Human XM_006724286.3

PREDICTED: Homo sapiens Rac family small GTPase 2 (RAC2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RAC2 (5880)
Length:
735
CDS:
112..597

Additional Resources:

NCBI RefSeq record:
XM_006724286.3
NBCI Gene record:
RAC2 (5880)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006724286.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047275 GACAAGGACACCATCGAGAAA pLKO.1 475 CDS 100% 4.950 3.465 N RAC2 n/a
2 TRCN0000291828 GACAAGGACACCATCGAGAAA pLKO_005 475 CDS 100% 4.950 3.465 N RAC2 n/a
3 TRCN0000047276 TCTCATCAGCTACACCACCAA pLKO.1 168 CDS 100% 2.640 1.848 N RAC2 n/a
4 TRCN0000291757 TCTCATCAGCTACACCACCAA pLKO_005 168 CDS 100% 2.640 1.848 N RAC2 n/a
5 TRCN0000047274 CCACCGTGTTTGACAACTATT pLKO.1 212 CDS 100% 13.200 7.920 N RAC2 n/a
6 TRCN0000291829 CCACCGTGTTTGACAACTATT pLKO_005 212 CDS 100% 13.200 7.920 N RAC2 n/a
7 TRCN0000065345 CCAATGTGATGGTGGACAGTA pLKO.1 236 CDS 100% 4.950 2.970 N Rac2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006724286.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06832 pDONR223 100% 82.6% 75% None (many diffs) n/a
2 ccsbBroad304_06832 pLX_304 0% 82.6% 75% V5 (many diffs) n/a
3 TRCN0000467053 TCCTGGTGCGGGTACCTTCGAGCA pLX_317 42.4% 82.6% 75% V5 (many diffs) n/a
4 ccsbBroadEn_06833 pDONR223 100% 67.6% 69.5% None (many diffs) n/a
5 ccsbBroad304_06833 pLX_304 0% 67.6% 69.5% V5 (many diffs) n/a
6 TRCN0000468968 GCGGTTAAACATGACAGCCCCCAT pLX_317 71.4% 67.6% 69.5% V5 (many diffs) n/a
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