Construct: ORF TRCN0000468968
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007632.1_s317c1
- Derived from:
- ccsbBroadEn_06833
- DNA Barcode:
- GCGGTTAAACATGACAGCCCCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAC3 (5881)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468968
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5881 | RAC3 | Rac family small GTPase 3 | NM_005052.3 | 99.8% | 99.4% | 16T>G |
| 2 | human | 5881 | RAC3 | Rac family small GTPase 3 | NM_001316307.2 | 87% | 81.7% | 16T>G;448_451delGGTG;510_511ins70 |
| 3 | human | 5880 | RAC2 | Rac family small GTPase 2 | NM_002872.5 | 81.8% | 88% | (many diffs) |
| 4 | human | 5879 | RAC1 | Rac family small GTPase 1 | NM_006908.5 | 75.8% | 92.1% | (many diffs) |
| 5 | human | 5879 | RAC1 | Rac family small GTPase 1 | NM_018890.4 | 69.7% | 83.8% | (many diffs) |
| 6 | human | 5880 | RAC2 | Rac family small GTPase 2 | XM_006724286.3 | 67.6% | 69.5% | (many diffs) |
| 7 | mouse | 170758 | Rac3 | RAS-related C3 botulinum su... | NM_133223.4 | 89.2% | 99.4% | (many diffs) |
| 8 | mouse | 19354 | Rac2 | RAS-related C3 botulinum su... | NM_009008.3 | 80.9% | 87.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 642
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggccatcaag ggcgtggtgg tcggcgacgg cgccgtgggg aagacatgct 121 tgctgatcag ctacacgacc aacgccttcc ccggagagta catccccacc gtttttgaca 181 actactctgc caacgtgatg gtggacggga aaccagtcaa cttgggGCTG TGGGACACAG 241 CGGGTCAGGA GGACTACGAT CGGCTGCGGC CACTCTCCTA CCCCCAAACT GACGTCTTTC 301 TGATCTGCTT CTCTCTGGTG AGCCCGGCCT CCTTCGAGAA TGTTCGTGCC AAGTGGTACC 361 CGGAGGTGCG GCACCACTGC CCCCACACGC CCATCCTCCT GGTGGGCACC AAGCTGGACC 421 TCCGCGACGA CAAGGACACC ATTGAGCGGC TGCGGGACAA GAAGCTGGCA CCCATCACCT 481 ACCCACAGGG CCTGGCCATG GCCCGGGAGA TTGGCTCTGT GAAATACCTG GAGTGCTCAG 541 CCCTGACCCA GCGGGGCCTG AAGACAGTGT TTGACGAGGC GATCCGCGCG GTGCTCTGCC 601 CGCCCCCAGT GAAGAAGCCG GGGAAGAAGT GCACCGTCTT CTACCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAGCG GTTAAACATG ACAGCCCCCA TACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t