Transcript: Mouse XM_011238569.1

PREDICTED: Mus musculus family with sequence similarity 135, member A (Fam135a), transcript variant X12, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fam135a (68187)
Length:
4033
CDS:
81..2831

Additional Resources:

NCBI RefSeq record:
XM_011238569.1
NBCI Gene record:
Fam135a (68187)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011238569.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000264934 GTAAGTAGTGACACGATTAAA pLKO_005 552 CDS 100% 15.000 21.000 N Fam135a n/a
2 TRCN0000345671 TAAGTAGTGACACGATTAAAT pLKO_005 553 CDS 100% 15.000 21.000 N Fam135a n/a
3 TRCN0000264932 CGATCCTTATTCAGACTATAT pLKO_005 3131 3UTR 100% 13.200 10.560 N Fam135a n/a
4 TRCN0000190316 CGGAACACAATCTGGCATCTA pLKO.1 199 CDS 100% 4.950 3.960 N Fam135a n/a
5 TRCN0000283238 GACATTCGTTAGGCAATTTAA pLKO_005 2257 CDS 100% 15.000 10.500 N Fam135a n/a
6 TRCN0000345745 GATCCTTATTCAGACTATATC pLKO_005 3132 3UTR 100% 13.200 9.240 N Fam135a n/a
7 TRCN0000216603 GATGCTAAGTTTATGACATAT pLKO.1 2905 3UTR 100% 13.200 9.240 N Fam135a n/a
8 TRCN0000136155 GCCCGCATTGAAATGTGTAAA pLKO.1 2580 CDS 100% 13.200 9.240 N FAM135A n/a
9 TRCN0000201523 CCTCATTTGGATGCTCCCTTT pLKO.1 1134 CDS 100% 4.050 2.835 N Fam135a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011238569.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08746 pDONR223 100% 59.3% 54.2% None (many diffs) n/a
2 ccsbBroad304_08746 pLX_304 0% 59.3% 54.2% V5 (many diffs) n/a
3 TRCN0000471886 TCACCTAATACCTTCTTGCACCTA pLX_317 10.3% 59.3% 54.2% V5 (many diffs) n/a
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