Construct: ORF ccsbBroad304_08746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015405.1_s304c1, BRDN0000401936
- Derived from:
- ccsbBroadEn_08746
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM135A (57579)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_08746
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57579 | FAM135A | family with sequence simila... | NM_001105531.2 | 99.9% | 99.9% | 3137A>G |
| 2 | human | 57579 | FAM135A | family with sequence simila... | NM_001330997.2 | 99.9% | 99.9% | 3137A>G |
| 3 | human | 57579 | FAM135A | family with sequence simila... | NM_001331001.2 | 99.9% | 99.9% | 3137A>G |
| 4 | human | 57579 | FAM135A | family with sequence simila... | NM_001331002.2 | 99.9% | 99.9% | 3137A>G |
| 5 | human | 57579 | FAM135A | family with sequence simila... | NM_001330998.2 | 98% | 97.9% | 823_900del;3215A>G |
| 6 | human | 57579 | FAM135A | family with sequence simila... | NM_001331003.2 | 98% | 97.9% | 823_900del;3215A>G |
| 7 | human | 57579 | FAM135A | family with sequence simila... | NM_001331000.2 | 97.4% | 97.4% | 0_1ins99;3038A>G |
| 8 | human | 57579 | FAM135A | family with sequence simila... | NM_020819.4 | 94.8% | 94.7% | 76_77ins129;694_771del;3086A>G |
| 9 | human | 57579 | FAM135A | family with sequence simila... | NM_001351608.1 | 93.4% | 93.4% | 0_1ins258;2879A>G |
| 10 | human | 57579 | FAM135A | family with sequence simila... | NM_001162529.2 | 87% | 86.9% | 1257_1844del;3725A>G |
| 11 | human | 57579 | FAM135A | family with sequence simila... | NM_001351600.1 | 87% | 86.9% | 1257_1844del;3725A>G |
| 12 | human | 57579 | FAM135A | family with sequence simila... | NM_001351602.1 | 87% | 86.9% | 1257_1844del;3725A>G |
| 13 | human | 57579 | FAM135A | family with sequence simila... | NM_001351607.1 | 87% | 86.9% | 1257_1844del;3725A>G |
| 14 | human | 57579 | FAM135A | family with sequence simila... | XM_017011127.1 | 87% | 86.9% | 1257_1844del;3725A>G |
| 15 | human | 57579 | FAM135A | family with sequence simila... | NM_001330996.2 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 16 | human | 57579 | FAM135A | family with sequence simila... | NM_001330999.2 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 17 | human | 57579 | FAM135A | family with sequence simila... | NM_001351599.1 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 18 | human | 57579 | FAM135A | family with sequence simila... | XM_011535993.3 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 19 | human | 57579 | FAM135A | family with sequence simila... | XM_011535994.2 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 20 | human | 57579 | FAM135A | family with sequence simila... | XM_011535995.3 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 21 | human | 57579 | FAM135A | family with sequence simila... | XM_011535996.2 | 85.5% | 85.5% | 823_900del;1335_1922del;3803A>G |
| 22 | human | 57579 | FAM135A | family with sequence simila... | NM_001331005.2 | 84.8% | 84.8% | 0_1ins99;1158_1745del;3626A>G |
| 23 | human | 57579 | FAM135A | family with sequence simila... | XM_005248749.1 | 83.9% | 70.9% | (many diffs) |
| 24 | human | 57579 | FAM135A | family with sequence simila... | XM_011535997.2 | 83.4% | 83.3% | (many diffs) |
| 25 | human | 57579 | FAM135A | family with sequence simila... | NM_001330995.2 | 82.9% | 82.9% | 1518_1519ins672;2465A>G |
| 26 | human | 57579 | FAM135A | family with sequence simila... | NM_001331006.2 | 81.3% | 81.3% | 823_900del;1596_1597ins672;2543A>G |
| 27 | human | 57579 | FAM135A | family with sequence simila... | NM_001331004.2 | 76.3% | 68% | (many diffs) |
| 28 | human | 57579 | FAM135A | family with sequence simila... | XM_017011136.1 | 76.3% | 68% | (many diffs) |
| 29 | human | 57579 | FAM135A | family with sequence simila... | NM_001351609.1 | 74.9% | 74.9% | (many diffs) |
| 30 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496234.2 | 86.3% | 83.9% | (many diffs) |
| 31 | mouse | 68187 | Fam135a | family with sequence simila... | XM_017322275.1 | 85% | 81.3% | (many diffs) |
| 32 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496233.2 | 83.4% | 79.8% | (many diffs) |
| 33 | mouse | 68187 | Fam135a | family with sequence simila... | NM_026604.4 | 75.2% | 73.1% | (many diffs) |
| 34 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496232.3 | 74.1% | 70.9% | (many diffs) |
| 35 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496230.3 | 73.9% | 71.8% | (many diffs) |
| 36 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496231.3 | 73.9% | 71.8% | (many diffs) |
| 37 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496229.2 | 72.9% | 69.7% | (many diffs) |
| 38 | mouse | 68187 | Fam135a | family with sequence simila... | XM_011238569.1 | 59.3% | 54.2% | (many diffs) |
| 39 | mouse | 68187 | Fam135a | family with sequence simila... | XM_006496235.2 | 58.8% | 55.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4026
- ORF length:
- 3957
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactgaagtt caagcaatgg tagaattctc tgtggagcta aacaagttct 121 acaatgtgga tttgtttcag agaggttttt accagattcg tgcttctatg aaaattccat 181 caagaattcc ccacagagta gaagctagtt tgttgcatgc aacaggtatg actttagcct 241 ttccagcctc agttcatgat tctctaattt gcagtaaaac atttcaaatt ttgtacaaaa 301 atgaagaggt tgttttaaat gatgttatga tcttcaaagt aaaaatgcta ttggatgaaa 361 gaaagattga agaaaccctt gaggaaatga attttctatt atccttggat ctacacttca 421 cagatggaga ttattcggca gatgatctga atgccttgca actaataagt agccgaacat 481 tgaagctgca ctttagcccc catagaggcc ttcatcatca tgttaatgtt atgtttgatt 541 acttccacct ttctgttgtg tctgttacag ttcatgcatc attggttgca ctacaccagc 601 cactaataag ctttcctcgc cctgtgaaga caacttggtt aaatagaaat gcaccagcac 661 aaaacaaaga ttccgtgatt cctactcttg aaagtgtggt ctttggtatt aactacacaa 721 aacagttatc accagatggt tgtagcttca tcattgcaga ctccttccta catcatgcgt 781 atcgttttca ttatacactt tgtgccactt tgctgctagc cttcaaggga ttgcacagct 841 acttcattac agtaacagaa gagattcctt cttgtcagaa actagaactg gaggaaatgg 901 atgtagaagc tcgacttact gaactatgtg aagaagttaa gaaaatagag aatcctgatg 961 aactggcaga acttataaat atgaatcttg cgcaactttg ctcacttttg atggctttat 1021 ggggacagtt tctggaagtt ataacgctac acgaagaact aagaatatta ttagcacaag 1081 agcaccatac tttgagggta cgcagatttt ctgaggcatt cttttgtttt gagcatccaa 1141 gagaagctgc cattgcatac caggaacttc atgctcagag tcatctacag atgtgcaccg 1201 ctatcaaaaa tacttccttc tgcagttctc ttccaccctt acctattgaa tgtagtgaat 1261 tagatggaga tctcaattca ttacctataa tctttgaaga taggtattta gattcagtta 1321 ctgaaggtaa acttgatatc tcccaggacg atagtgaaat tacacaaatg gaacacaatc 1381 tggcatccag aaggtcatca gacgattgcc atgatcatca aacaacccca tctttgggag 1441 ttagaacaat tgaaataaag cccagtaata aagatccttt cagtggagag aatataactg 1501 tcaaactagg accttggaca gagcttcgac aagaggaaat acttgtggat aatttactac 1561 ccaactttga gtccttagaa tctaatggta aatctaaatc tatagaaata acatttgaaa 1621 aggaagcttt gcaagaagca aagtgtcttt ctattggaga atcattaact aaattacgaa 1681 gtaatctacc tgccccttct acaaaagaat atcatgttgt agtaagtgga gatacaatta 1741 agttaccaga tattagtgcc acatatgcct catctagatt ttcagattca ggtgttgaaa 1801 gtgaaccgag ttcttttgcg acacatccaa acactgattt agtctttgaa actgtgcaag 1861 ggcaaggtcc ttgcaatagt gaaagattat ttcctcagct tttgatgaaa cctgattata 1921 atgtaaaatt ttcattagga aatcattgta ctgagagtac aagtgctata agtgaaatac 1981 agtcatcttt gacatccata aactctctac cctccgatga tgaactgtca cctgatgaaa 2041 attctaagaa atctgttgta cctgaatgcc atctaaatga tagcaaaact gtattaaatc 2101 taggaacgac tgatttgcca aaatgtgatg atactaaaaa gtcaagtatc actttgcaac 2161 agcagagtgt tgtattttca gggaacttgg acaatgaaac tgtagcaata cattccttaa 2221 attcaagcat taaagaccct ttacaatttg ttttttcaga tgaagagact tccagtgatg 2281 tgaaaagtag ttgcagctcc aaacctaact tggatactat gtgtaaaggc ttccagagtc 2341 ctgataaatc taataactct acagggacag caattacatt aaattcaaaa ctgatttgtt 2401 taggcactcc ttgtgtcatt tcaggttcca tttctagtaa tacagatgtt agtgaagata 2461 gaactatgaa aaaaaatagt gatgtattaa atctcacaca gatgtattca gaaatcccta 2521 cagttgaaag tgaaactcat ctgggtacaa gtgatccttt ttcagccagt actgatatag 2581 taaagcaagg gcttgtggaa aattattttg gttctcaaag cagtacggat atttctgaca 2641 catgtgctgt tagctacagc aatgcactta gccctcagaa ggaaacttct gaaaaagaaa 2701 ttagtaatct tcagcaggaa caggataaag aggatgagga ggaagagcag gatcaacaaa 2761 tggttcaaaa tgggtactat gaagaaacag attattcagc tttggatgga acaataaatg 2821 ctcactatac aagcagagat gaactaatgg aagaaagact tacaaaatct gaaaaaataa 2881 acagtgacta tctgagagat ggtataaaca tgcctactgt ctgtacttct ggttgtttgt 2941 ccttcccgtc tgcaccacga gagtctcctt gtaatgttaa atattcttcc aaaagtaaat 3001 ttgatgccat tacaaagcag ccaagcagta cttcttacaa cttcacttct tcgatttcct 3061 ggtatgaaag ttcaccaaaa cctcaaatac aagccttcct tcaggcaaaa gaagaactga 3121 agctactaaa acttcctggg ttcatgtaca gtgaagttcc tctgctggca tcctcagtac 3181 cttattttag tgtagaagaa gagggtggtt ctgaagatgg agtacatctg attgtctgtg 3241 tgcacggttt agatggaaac agtgcagatc tccgattagt aaaaacttac attgaacttg 3301 gattgcctgg gggaagaatt gattttctta tgtctgagag aaatcagaat gatacttttg 3361 ctgattttga tagcatgact gatcgtcttt tggatgagat aatacagtat attcagatat 3421 atagtctaac agtctcaaaa ataagcttta ttggacattc gttgggcaat ttaataattc 3481 gttcagtgct tacaaggcca aggtttaaat attacctcaa caaacttcat acctttctgt 3541 ctctttctgg acctcacctt ggtacactct acaacagcag tgctcttgtt aatacaggtc 3601 tctggtttat gcagaaatgg aaaaaatcag gttcgctttt gcagctgaca tgtcgagatc 3661 actcagaccc tcgccaaact tttttatata agcttagtaa caaagcaggg cttcattatt 3721 tcaaaaatgt tgtgctagtg ggatccctac aggatcgcta tgttccttat cactctgccc 3781 gcattgaaat gtgtaaaaca gctttaaagg acaaacagtc aggacagatc tattcagaaa 3841 tgatccacaa cttgcttcga cccgttctgc aaagcaagga ctgtaatttg gttcgctata 3901 atgtcatcaa tgcattgccc aatacagctg attcactcat tgggagagct gcacatatag 3961 ctgttcttga ttcggaaata tttttagaga aattctttct ggttgctgcc ctcaaatatt 4021 tccaattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 4081 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 4141 acctctggat ta