Transcript: Mouse XM_011239316.2

PREDICTED: Mus musculus potassium voltage-gated channel, subfamily H (eag-related), member 7 (Kcnh7), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kcnh7 (170738)
Length:
6491
CDS:
344..2845

Additional Resources:

NCBI RefSeq record:
XM_011239316.2
NBCI Gene record:
Kcnh7 (170738)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011239316.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412860 AGACTTGATTGTGGATATTAT pLKO_005 616 CDS 100% 15.000 21.000 N Kcnh7 n/a
2 TRCN0000423189 AGTTCTGGACCGTCCATTAAA pLKO_005 1067 CDS 100% 15.000 21.000 N Kcnh7 n/a
3 TRCN0000430100 GGGAACGTTTCTGCAATTATT pLKO_005 1232 CDS 100% 15.000 21.000 N Kcnh7 n/a
4 TRCN0000021346 CCCTTTGAATGTGGTAGACTT pLKO.1 601 CDS 100% 4.950 3.465 N KCNH7 n/a
5 TRCN0000068448 CCTCCATTGATGATGAGCAAA pLKO.1 2070 CDS 100% 4.950 3.465 N Kcnh7 n/a
6 TRCN0000068452 CCTGCATCTGAACCAGACTTT pLKO.1 1453 CDS 100% 4.950 3.465 N Kcnh7 n/a
7 TRCN0000068450 CCTGAATTTCTCGACCTCGAA pLKO.1 2582 CDS 100% 2.640 1.848 N Kcnh7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011239316.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04505 pDONR223 100% 28.5% 29.1% None (many diffs) n/a
2 ccsbBroad304_04505 pLX_304 0% 28.5% 29.1% V5 (many diffs) n/a
3 TRCN0000473323 TAGAGGCGTACTTAGTCACGGCCG pLX_317 21.4% 28.5% 29.1% V5 (many diffs) n/a
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