Transcript: Mouse XM_011239840.2

PREDICTED: Mus musculus methyltransferase like 15 (Mettl15), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mettl15 (76894)
Length:
1464
CDS:
145..906

Additional Resources:

NCBI RefSeq record:
XM_011239840.2
NBCI Gene record:
Mettl15 (76894)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011239840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000097407 CATTCCTTAGAAGATCGTATT pLKO.1 649 CDS 100% 10.800 7.560 N Mettl15 n/a
2 TRCN0000097405 GCGGAGGCATTGCTAATGAAA pLKO.1 130 5UTR 100% 5.625 3.938 N Mettl15 n/a
3 TRCN0000097404 CCATATTTCATACAGTGGAAA pLKO.1 1232 3UTR 100% 4.950 3.465 N Mettl15 n/a
4 TRCN0000097406 GCTACAAAGATCCACCCACAT pLKO.1 504 CDS 100% 4.050 2.835 N Mettl15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011239840.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13367 pDONR223 100% 32% 16.7% None (many diffs) n/a
2 ccsbBroad304_13367 pLX_304 0% 32% 16.7% V5 (many diffs) n/a
3 TRCN0000473600 CGGGAGACTAGCCACCCGCCTACA pLX_317 54.2% 32% 16.7% V5 (many diffs) n/a
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