Transcript: Mouse XM_011239864.2

PREDICTED: Mus musculus oxysterol binding protein-like 6 (Osbpl6), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Osbpl6 (99031)
Length:
7888
CDS:
402..3230

Additional Resources:

NCBI RefSeq record:
XM_011239864.2
NBCI Gene record:
Osbpl6 (99031)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011239864.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000105096 CCGACAACATTTCTCGGCAAA pLKO.1 2026 CDS 100% 4.050 5.670 N Osbpl6 n/a
2 TRCN0000105098 GCCGCAGAGAAACAAAGAGTT pLKO.1 3030 CDS 100% 4.950 3.960 N Osbpl6 n/a
3 TRCN0000105095 CCCGCAAAGTATTCCTAATAA pLKO.1 4766 3UTR 100% 15.000 10.500 N Osbpl6 n/a
4 TRCN0000105097 GCAGGCTAACTGTGTAGATAT pLKO.1 1292 CDS 100% 13.200 9.240 N Osbpl6 n/a
5 TRCN0000161428 GCAGGCTAACTGTGTAGATAT pLKO.1 1292 CDS 100% 13.200 9.240 N OSBPL6 n/a
6 TRCN0000159097 GCTGCAACCATATTCCTTAAA pLKO.1 3426 3UTR 100% 13.200 9.240 N OSBPL6 n/a
7 TRCN0000105099 GCACAGAAAGTGCATTCTCTT pLKO.1 1545 CDS 100% 4.950 3.465 N Osbpl6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011239864.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13041 pDONR223 100% 47.5% 49.3% None (many diffs) n/a
2 ccsbBroad304_13041 pLX_304 0% 47.5% 49.3% V5 (many diffs) n/a
3 TRCN0000467518 TAACCGGAATGGGGTTTTACGCCT pLX_317 14.1% 47.5% 49.3% V5 (many diffs) n/a
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