Transcript: Mouse XM_011245359.1

PREDICTED: Mus musculus S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Skp2 (27401)
Length:
3207
CDS:
234..1538

Additional Resources:

NCBI RefSeq record:
XM_011245359.1
NBCI Gene record:
Skp2 (27401)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011245359.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088760 GCAGATTAACTGCGCCTATTT pLKO.1 1421 CDS 100% 13.200 18.480 N Skp2 n/a
2 TRCN0000302380 GCAGATTAACTGCGCCTATTT pLKO_005 1421 CDS 100% 13.200 18.480 N Skp2 n/a
3 TRCN0000088758 CGTGCTACAATCCATTTCTAT pLKO.1 2837 3UTR 100% 5.625 7.875 N Skp2 n/a
4 TRCN0000302319 CGTGCTACAATCCATTTCTAT pLKO_005 2837 3UTR 100% 5.625 7.875 N Skp2 n/a
5 TRCN0000088762 CCTGTCGAACTCAGTGATAAA pLKO.1 824 CDS 100% 13.200 10.560 N Skp2 n/a
6 TRCN0000302379 CCTGTCGAACTCAGTGATAAA pLKO_005 824 CDS 100% 13.200 10.560 N Skp2 n/a
7 TRCN0000088761 GCAAGACTTCTGAACTGCTAT pLKO.1 343 CDS 100% 4.950 3.465 N Skp2 n/a
8 TRCN0000302320 GCAAGACTTCTGAACTGCTAT pLKO_005 343 CDS 100% 4.950 3.465 N Skp2 n/a
9 TRCN0000088759 GCCTCGACTTAAGTGACAGTA pLKO.1 1204 CDS 100% 4.950 3.465 N Skp2 n/a
10 TRCN0000302321 GCCTCGACTTAAGTGACAGTA pLKO_005 1204 CDS 100% 4.950 3.465 N Skp2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011245359.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489546 CCGTCCCTATGTCACTGCTATTAC pLX_317 30.7% 82.9% 83.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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