Transcript: Mouse XM_011247713.2

PREDICTED: Mus musculus dachshund 2 (Drosophila) (Dach2), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dach2 (93837)
Length:
7120
CDS:
339..2318

Additional Resources:

NCBI RefSeq record:
XM_011247713.2
NBCI Gene record:
Dach2 (93837)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011247713.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000436481 ATATCCATCATGGAGTTATTT pLKO_005 6734 3UTR 100% 15.000 21.000 N Dach2 n/a
2 TRCN0000108579 GATAAGATACAATCTCCACTT pLKO.1 1158 CDS 100% 4.050 3.240 N Dach2 n/a
3 TRCN0000423706 AGCTGAAGAGACTCGACATAT pLKO_005 694 CDS 100% 13.200 9.240 N Dach2 n/a
4 TRCN0000431313 TAATCTTTGTCCTGGTATTAG pLKO_005 6857 3UTR 100% 13.200 9.240 N Dach2 n/a
5 TRCN0000422179 TACTTGTGATTCCAGTATATC pLKO_005 6706 3UTR 100% 13.200 9.240 N Dach2 n/a
6 TRCN0000415375 AGACTCTGTTGACCAACATTC pLKO_005 1900 CDS 100% 10.800 7.560 N DACH2 n/a
7 TRCN0000108578 CCTCGCAGATGGATCATCATT pLKO.1 1567 CDS 100% 5.625 3.938 N Dach2 n/a
8 TRCN0000020813 AGGAGGTAACTATTACTGTTT pLKO.1 6623 3UTR 100% 4.950 3.465 N DACH2 n/a
9 TRCN0000108576 CCAACAATCGAGAAGAGCTTA pLKO.1 1609 CDS 100% 4.950 3.465 N Dach2 n/a
10 TRCN0000108577 CCCTAACATGATCAACAGTTT pLKO.1 455 CDS 100% 4.950 3.465 N Dach2 n/a
11 TRCN0000020811 CCATTTATGATGATGCCTCAT pLKO.1 1302 CDS 100% 4.050 2.835 N DACH2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011247713.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13061 pDONR223 100% 58.6% 57.9% None (many diffs) n/a
2 ccsbBroad304_13061 pLX_304 0% 58.6% 57.9% V5 (many diffs) n/a
3 TRCN0000473331 TGCTGATAAACTCCCGCTAAAGCA pLX_317 35.9% 58.6% 57.9% V5 (many diffs) n/a
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