Construct: ORF TRCN0000473331

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008208.1_s317c1
Derived from:
ccsbBroadEn_13061
DNA Barcode:
TGCTGATAAACTCCCGCTAAAGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DACH2 (117154)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473331
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 117154 DACH2 dachshund family transcript... NM_001139515.1 91.7% 91.3% (many diffs)
2 human 117154 DACH2 dachshund family transcript... XM_017029255.2 76.3% 73.9% (many diffs)
3 human 117154 DACH2 dachshund family transcript... XM_017029254.2 75.1% 72.7% (many diffs)
4 human 117154 DACH2 dachshund family transcript... NM_053281.3 74.9% 72.2% (many diffs)
5 human 117154 DACH2 dachshund family transcript... NM_001139514.1 73.7% 71.2% (many diffs)
6 human 117154 DACH2 dachshund family transcript... XM_017029256.2 73.7% 71.2% (many diffs)
7 human 117154 DACH2 dachshund family transcript... XM_011530846.3 73.7% 71% (many diffs)
8 human 117154 DACH2 dachshund family transcript... XM_011530848.3 72.3% 69.5% (many diffs)
9 human 117154 DACH2 dachshund family transcript... XM_011530847.3 71.1% 68.4% (many diffs)
10 mouse 93837 Dach2 dachshund 2 (Drosophila) NM_001289733.1 76.4% 77.8% (many diffs)
11 mouse 93837 Dach2 dachshund 2 (Drosophila) NM_001289732.1 73.4% 74.4% (many diffs)
12 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247716.1 73.4% 74.4% (many diffs)
13 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_006528416.3 65.6% 65.4% (many diffs)
14 mouse 93837 Dach2 dachshund 2 (Drosophila) NM_001289734.1 64.7% 66.4% (many diffs)
15 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247717.2 64.7% 66.4% (many diffs)
16 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247714.1 64.6% 63.9% (many diffs)
17 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247715.1 64.6% 63.9% (many diffs)
18 mouse 93837 Dach2 dachshund 2 (Drosophila) NM_033605.2 64.5% 63.9% (many diffs)
19 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_006528413.3 63.5% 63% (many diffs)
20 mouse 93837 Dach2 dachshund 2 (Drosophila) NM_001142570.1 63.3% 63.2% (many diffs)
21 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247712.2 61.7% 61.3% (many diffs)
22 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247711.2 61.6% 60.9% (many diffs)
23 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247710.2 60.7% 60% (many diffs)
24 mouse 93837 Dach2 dachshund 2 (Drosophila) XM_011247713.2 58.6% 57.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1464
ORF length:
1395
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccagagttt cgctctgttg cccaggctgc agtgcagtgg tgtgatcttg
121gctcgctgca acttccgcct cccaggttca agggattctt gtgcctcagc ctcccgagta
181gctggaatta cagttcaaga cccggcaggc cccctaagcg ttctttggga gtgttgcagg
241aaaatgcccg ccttctgacc catgcagtcc caggcctctt atcgccagga cttatcactc
301cgacaggtat aacagctgca gcgatggctg aggcgatgaa acttcagaag atgaagctta
361tggctatgaa cactcttcag ggaaatggaa gccaaaatgg gaccgaatca gagcctgatg
421atcttaattc taacacaggt ggaagtgaat cctcctggga taaagataag atgcagtctc
481catttgctgc acctggaccc caacatggaa ttgctcatgc agccctagct ggccagccag
541gcattggggg tgctccaacc ctcaatccac tgcagcagaa ccacctgcta accaatagac
601tggatctgcc atttatgatg atgcctcatc ccctacttcc agtcagctta cctcctgcat
661cagttgccat ggcaatgaat cagatgaacc atctcaatac tattgccaac atggctgctg
721cagcacagat tcacagtcca ctctccagag ctggtacctc tgttataaag gagcggatcc
781cagagagtcc ttctcctgct ccttctctag aagagaatca tcgtcctggg agccagacct
841cttcccacac cagcagcagt gtgtccagct ctccctctca gatggatcat catttggaaa
901gaatggaaga ggtaccagtt caaattccaa taatgaagtc acccttggac aagatacagc
961tgacTCCTGG GCAGGCATTG CCCGCTGGAT TCCCTGGACC ATTCATTTTT GCTGATAGTC
1021TGTCCTCCGT GGAGACTCTG TTGACCAACA TTCAGGGTCT GCTGAAAGTT GCTTTGGATA
1081ATGCTCGCAT CCAGGAGAAG CAGATTCAAC AAGAAAAGAA GGAGCTGCGA CTGGAGCTCT
1141ATAGAGAGAG AGAAATTAGA GAAAACCTTG AAAGACAACT TGCAGTTGAG CTTCAAAGCA
1201GAACTACTAT GCAAAAGCGC CTGAAGAAGG AGAAAAAAAC CAAGAGAAAA TTGCAGGAAG
1261CCTTGGAATT TGAATCAAAG CGCCGGGAGC AAGTGGAGCA GGCACTTAAG CAAGCCACCA
1321CTAGTGACAG TGGCCTGAGG ATGTTAAAAG ATACTGGAAT TCCAGATATT GAAATAGAAA
1381ACAATGGGAC TCCTCATGAT AGTGCTGCTA TGCAAGGAGG TAACTATTAC TGTTTAGAAA
1441TGGCACAACA GTTGTATTCA GCCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1501AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1561TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GCTGATAAAC
1621TCCCGCTAAA GCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1681att

Download FASTA (ORF) (Full)