Transcript: Mouse XM_011248726.1

PREDICTED: Mus musculus gamma-aminobutyric acid (GABA) A receptor, subunit beta 2 (Gabrb2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gabrb2 (14401)
Length:
7296
CDS:
75..1613

Additional Resources:

NCBI RefSeq record:
XM_011248726.1
NBCI Gene record:
Gabrb2 (14401)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011248726.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000102983 CCTAACAATGACCACAATCAA pLKO.1 920 CDS 100% 5.625 7.875 N Gabrb2 n/a
2 TRCN0000061395 CCTAGTAATATGTCGCTGGTT pLKO.1 162 CDS 100% 2.640 3.696 N GABRB2 n/a
3 TRCN0000196206 GCAGGATTCATTGCATCCGTT pLKO.1 7067 3UTR 100% 2.640 2.112 N Gabrb2 n/a
4 TRCN0000102980 GCCTACTTAGAGGGTTATTAT pLKO.1 1893 3UTR 100% 15.000 10.500 N Gabrb2 n/a
5 TRCN0000183322 GTCTTCACAATGTAGTGATTA pLKO.1 6965 3UTR 100% 13.200 9.240 N Gabrb2 n/a
6 TRCN0000436857 GGCCTATCCTGTGGTCCATTT pLKO_005 1735 3UTR 100% 10.800 7.560 N GABRB2 n/a
7 TRCN0000180307 CCACTTGAATCAAGCCAACAT pLKO.1 6725 3UTR 100% 4.950 3.465 N Gabrb2 n/a
8 TRCN0000102981 CCATCAATTCTGATTACCATT pLKO.1 828 CDS 100% 4.950 3.465 N Gabrb2 n/a
9 TRCN0000102984 CCTGACACCTACTTCCTGAAT pLKO.1 426 CDS 100% 4.950 3.465 N Gabrb2 n/a
10 TRCN0000183608 GAATTGTTCCTGCATAGTTCT pLKO.1 6911 3UTR 100% 4.950 3.465 N Gabrb2 n/a
11 TRCN0000102982 GCCATCAATTCTGATTACCAT pLKO.1 827 CDS 100% 3.000 2.100 N Gabrb2 n/a
12 TRCN0000182895 CACTCAAACAAAGTTGCTTAT pLKO.1 6827 3UTR 100% 10.800 6.480 N Gabrb2 n/a
13 TRCN0000180119 CCCATCACTTTCCACTTGAAT pLKO.1 6714 3UTR 100% 5.625 3.375 N Gabrb2 n/a
14 TRCN0000180803 GCCAACATTTCCATCAGCTTT pLKO.1 6738 3UTR 100% 4.950 3.465 N Gabrb2 n/a
15 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5872 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011248726.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00607 pDONR223 100% 84.5% 92.3% None (many diffs) n/a
2 ccsbBroad304_00607 pLX_304 0% 84.5% 92.3% V5 (many diffs) n/a
3 TRCN0000473027 ATTAGACTACACAGATAACTCATG pLX_317 32% 84.5% 92.3% V5 (many diffs) n/a
4 ccsbBroadEn_06243 pDONR223 100% 84.3% 92.1% None (many diffs) n/a
5 ccsbBroad304_06243 pLX_304 0% 84.3% 92.1% V5 (many diffs) n/a
6 TRCN0000477872 CGCTTCTCACAGATCCAGCAAGTC pLX_317 31% 84.3% 92.1% V5 (many diffs) n/a
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