Transcript: Human XM_011510403.3

PREDICTED: Homo sapiens ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ASAP2 (8853)
Length:
5496
CDS:
83..3145

Additional Resources:

NCBI RefSeq record:
XM_011510403.3
NBCI Gene record:
ASAP2 (8853)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011510403.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379573 GAAATAAGCGGAGCGGAAATT pLKO_005 644 CDS 100% 13.200 18.480 N ASAP2 n/a
2 TRCN0000379717 GATCCTGCCTCTACGGAATTA pLKO_005 3421 3UTR 100% 13.200 18.480 N ASAP2 n/a
3 TRCN0000353707 GCGGGTGAAAGCGCTCTATAA pLKO_005 2965 CDS 100% 13.200 18.480 N ASAP2 n/a
4 TRCN0000381484 AGCTACAGATGTGCGAGTATC pLKO_005 699 CDS 100% 10.800 15.120 N ASAP2 n/a
5 TRCN0000029753 CCGGTGTCATTTGTGCACTTT pLKO.1 3113 CDS 100% 4.950 3.960 N ASAP2 n/a
6 TRCN0000330986 CCGGTGTCATTTGTGCACTTT pLKO_005 3113 CDS 100% 4.950 3.960 N ASAP2 n/a
7 TRCN0000331049 GTTGATACAGCTTCGAGATAT pLKO_005 910 CDS 100% 13.200 9.240 N ASAP2 n/a
8 TRCN0000330988 TATGTTCCTGTTTCGTTATTG pLKO_005 3174 3UTR 100% 13.200 9.240 N ASAP2 n/a
9 TRCN0000380947 TTCGTCAGAGCACAGCTTATA pLKO_005 984 CDS 100% 13.200 9.240 N ASAP2 n/a
10 TRCN0000093246 CGAGACCCATGAGGACTACAA pLKO.1 118 CDS 100% 4.950 3.465 N 1700030C10Rik n/a
11 TRCN0000029752 CCCTTTGGACAGTTTGCTGAA pLKO.1 490 CDS 100% 4.050 2.835 N ASAP2 n/a
12 TRCN0000029750 CCTGGATAAACAGACAGGGAA pLKO.1 1963 CDS 100% 2.640 1.848 N ASAP2 n/a
13 TRCN0000029749 GCCTCAAACCTTCCATTGAAA pLKO.1 834 CDS 100% 5.625 3.375 N ASAP2 n/a
14 TRCN0000331046 GCCTCAAACCTTCCATTGAAA pLKO_005 834 CDS 100% 5.625 3.375 N ASAP2 n/a
15 TRCN0000243553 GAGACCCATGAGGACTACAAG pLKO_005 119 CDS 100% 4.950 2.970 N Gm9222 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011510403.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07314 pDONR223 100% 94.1% 93.9% None (many diffs) n/a
2 ccsbBroad304_07314 pLX_304 0% 94.1% 93.9% V5 (many diffs) n/a
3 TRCN0000465586 TGACTTTAATCGCTTTACTTAGAA pLX_317 12.1% 94.1% 93.9% V5 (many diffs) n/a
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