Construct: ORF TRCN0000465586
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005184.1_s317c1
- Derived from:
- ccsbBroadEn_07314
- DNA Barcode:
- TGACTTTAATCGCTTTACTTAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASAP2 (8853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465586
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | NM_001135191.1 | 99.8% | 99.7% | 256T>C;1104C>T;2244G>C |
| 2 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_006711902.1 | 99.5% | 99.4% | (many diffs) |
| 3 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510407.2 | 98.4% | 98.3% | (many diffs) |
| 4 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | NM_003887.3 | 95.4% | 95.2% | (many diffs) |
| 5 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_006711898.1 | 95.1% | 94.9% | (many diffs) |
| 6 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510404.2 | 94.3% | 94.1% | (many diffs) |
| 7 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510403.3 | 94.1% | 93.9% | (many diffs) |
| 8 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510406.2 | 91.9% | 90.8% | (many diffs) |
| 9 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510405.3 | 91.6% | 91.4% | (many diffs) |
| 10 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_006711901.3 | 91.1% | 90.8% | (many diffs) |
| 11 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510408.3 | 86.1% | 80.9% | (many diffs) |
| 12 | human | 8853 | ASAP2 | ArfGAP with SH3 domain, ank... | XM_011510409.1 | 83.4% | 83.3% | (many diffs) |
| 13 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | NM_001004364.2 | 86.7% | 94.6% | (many diffs) |
| 14 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | NM_001098168.1 | 86.5% | 94.3% | (many diffs) |
| 15 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | XM_006515048.2 | 82.9% | 90.3% | (many diffs) |
| 16 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | XM_006515047.2 | 82.6% | 90% | (many diffs) |
| 17 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | NM_001135192.1 | 82.3% | 89.7% | (many diffs) |
| 18 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | XM_030246641.1 | 71.9% | 78.5% | (many diffs) |
| 19 | mouse | 211914 | Asap2 | ArfGAP with SH3 domain, ank... | XR_001780443.2 | 40.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2949
- ORF length:
- 2883
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggaccagatc tccgtgtcgg aattcgtggc cgagacccat gaggactaca 121 aggcgcccac ggcctccagc ttcaccaccc gcacggcgca gtgccggaac actgtggcgg 181 ccatcgagga ggctttggac gtggaccgga tggttcttta caaaatgaag aaatccgtga 241 aagcaatcaa cagctctggg ctggctcacg tggaaaatga agagcagtac acccaggctc 301 tggagaagtt tggcggcaac cgtgtatgca gagatgaccc agatttagga agtgcgttcc 361 tgaagttctc agtgtttaca aaggagttga cagcactttt caaaaacctg attcagaata 421 tgaacaacat aatctccttc cctttggaca gtttgctgaa gggggacctg aaaggagtga 481 aaggggatct gaaaaagcct tttgataaag cttggaagga ctatgaaaca aaaataacca 541 agatagaaaa ggagaaaaag gaacacgcca agctccatgg gatgattcgg actgaaataa 601 gcggagcgga aattgccgaa gagatggaaa aggagaggcg cttcttccag ctacagatgt 661 gcgagtatct gctgaaggtc aacgaaatca agattaaaaa gggagtagat ttacttcaga 721 atctgatcaa atactttcat gcccaatgca atttttttca ggatggactc aaagccgtgg 781 aaagcctcaa accttccatt gaaacgctgt ctacggatct tcacacgatc aaacaggccc 841 aggatgaaga aagaaggcag ttgatacagc ttcgagatat tttgaaatcc gcattgcagg 901 ttgaacagaa agaggactcc caaattcgtc agagcacagc ttatagctta catcagcctc 961 agggaaacaa ggaacatggg accgagcgga acggcagcct ctacaagaag agtgacggga 1021 tccgaaaagt gtggcagaaa aggaaatgtt cagttaaaaa tggttttctg accatatccc 1081 atggtaccgc taaccggcct cctgcaaagc tcaacctgct aacctgccag gtgaagacca 1141 accctgagga gaagaagtgc tttgacctta tttcacatga cagaacttac cactttcaag 1201 ctgaagatga acaggaatgt caaatatgga tgtctgtgct gcaaaatagc aaagaagaag 1261 ctttaaacaa tgcatttaag ggggatgaca atactggaga aaataacatc gtccaagaac 1321 tgacaaagga gatcatctca gaagtgcaga ggatgacggg caatgacgtc tgctgtgact 1381 gtggggcgcc agatcctaca tggctttcca ccaacctggg catcctgacc tgcatcgagt 1441 gttccggaat ccaccgagag ctgggggttc attattccag gatgcagtcc ctgaccttag 1501 atgtactggg aacatctgag ctgctgctcg ccaagaatat tgggaatgca ggctttaatg 1561 agatcatgga atgttgccta ccagctgagg actcagtcaa acccaaccca ggcagcgaca 1621 tgaatgcaag aaaggactac atcacagcca agtacatcga gaggagatac gcaaggaaga 1681 agcacgcgga taacgcggcg aagcttcaca gtctttgcga ggccgtcaaa acgagagata 1741 tttttggatt gctccaagct tatgctgatg gtgtggatct tacggaaaaa atcccactgg 1801 ccaacggaca tgagccggat gaaacggccc tccaccttgc agtcagatcc gtggatcgaa 1861 cctctcttca cattgtagac tttttagttc agaacagtgg gaacctggat aaacagacag 1921 ggaaaggcag cacagccctg cactactgct gcctgaccga caatgccgag tgcctcaagt 1981 tgctcctgcg ggggaaggcc tccatcgaga tagcaaacga gtcaggagag actccgctgg 2041 acattgccaa gcgcctcaag cacgagcact gtgaggagct gctgacccaa gccttatctg 2101 gaagatttaa ttctcacgtt cacgttgaat atgaatggcg actactccac gaagacctgg 2161 atgaaagtga tgacgacatg gatgagaaat tgcagcccag tcccaaccgg cgggaagacc 2221 ggcccatcag cttctaccag ctgggctcca accagcttca gtctaacgct gtatctttgg 2281 ccagagatgc tgcaaacctt gccaaggaca agcagagggc tttcatgccc agcatcttgc 2341 agaatgagac ttacggagcc ctcctgagtg gcagcccacc tcccgcccag cctgcagccc 2401 ccagcaccac cagcgccccc ccgcttcctc cacggaatgt tggcaaagat cccctgaccc 2461 ccacgccgcc cccacccgtt gccaagacgc ccagcgtaat ggaagccttg agccagccga 2521 gcaagcctgc cccgcctggg atctcacaga tcaggccccc acctctgccc ccacagccgc 2581 ccagccgcct cccgcagaag aagccTGCGC CGGGGGCTGA CAAGTCCACC CCACTGACCA 2641 ACAAAGGCCA ACCGAGAGGA CCTGTGGATC TCTCTGCAAC GGAAGCTCTG GGTCCTCTGT 2701 CCAATGCTAT GGTCCTGCAG CCCCCTGCAC CCATGCCTAG GAAGTCGCAG GCAACCAAGT 2761 TGAAGCCTAA GCGGGTGAAA GCGCTCTATA ACTGTGTGGC TGACAACCCC GATGAGCTCA 2821 CCTTCTCCGA GGGGGATGTG ATCATCGTGG ACGGGGAGGA GGACCAGGAG TGGTGGATTG 2881 GCCACATTGA TGGAGATCCT GGTCGCAAAG GCGCATTCCC GGTGTCATTT GTGCACTTTA 2941 TCGCTGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3001 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 3061 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGACTT TAATCGCTTT ACTTAGAAAC 3121 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt