Construct: ORF TRCN0000465586

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005184.1_s317c1
Derived from:
ccsbBroadEn_07314
DNA Barcode:
TGACTTTAATCGCTTTACTTAGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASAP2 (8853)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465586
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8853 ASAP2 ArfGAP with SH3 domain, ank... NM_001135191.1 99.8% 99.7% 256T>C;1104C>T;2244G>C
2 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_006711902.1 99.5% 99.4% (many diffs)
3 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510407.2 98.4% 98.3% (many diffs)
4 human 8853 ASAP2 ArfGAP with SH3 domain, ank... NM_003887.3 95.4% 95.2% (many diffs)
5 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_006711898.1 95.1% 94.9% (many diffs)
6 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510404.2 94.3% 94.1% (many diffs)
7 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510403.3 94.1% 93.9% (many diffs)
8 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510406.2 91.9% 90.8% (many diffs)
9 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510405.3 91.6% 91.4% (many diffs)
10 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_006711901.3 91.1% 90.8% (many diffs)
11 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510408.3 86.1% 80.9% (many diffs)
12 human 8853 ASAP2 ArfGAP with SH3 domain, ank... XM_011510409.1 83.4% 83.3% (many diffs)
13 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... NM_001004364.2 86.7% 94.6% (many diffs)
14 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... NM_001098168.1 86.5% 94.3% (many diffs)
15 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... XM_006515048.2 82.9% 90.3% (many diffs)
16 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... XM_006515047.2 82.6% 90% (many diffs)
17 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... NM_001135192.1 82.3% 89.7% (many diffs)
18 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... XM_030246641.1 71.9% 78.5% (many diffs)
19 mouse 211914 Asap2 ArfGAP with SH3 domain, ank... XR_001780443.2 40.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2949
ORF length:
2883
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc ggaccagatc tccgtgtcgg aattcgtggc cgagacccat gaggactaca
121aggcgcccac ggcctccagc ttcaccaccc gcacggcgca gtgccggaac actgtggcgg
181ccatcgagga ggctttggac gtggaccgga tggttcttta caaaatgaag aaatccgtga
241aagcaatcaa cagctctggg ctggctcacg tggaaaatga agagcagtac acccaggctc
301tggagaagtt tggcggcaac cgtgtatgca gagatgaccc agatttagga agtgcgttcc
361tgaagttctc agtgtttaca aaggagttga cagcactttt caaaaacctg attcagaata
421tgaacaacat aatctccttc cctttggaca gtttgctgaa gggggacctg aaaggagtga
481aaggggatct gaaaaagcct tttgataaag cttggaagga ctatgaaaca aaaataacca
541agatagaaaa ggagaaaaag gaacacgcca agctccatgg gatgattcgg actgaaataa
601gcggagcgga aattgccgaa gagatggaaa aggagaggcg cttcttccag ctacagatgt
661gcgagtatct gctgaaggtc aacgaaatca agattaaaaa gggagtagat ttacttcaga
721atctgatcaa atactttcat gcccaatgca atttttttca ggatggactc aaagccgtgg
781aaagcctcaa accttccatt gaaacgctgt ctacggatct tcacacgatc aaacaggccc
841aggatgaaga aagaaggcag ttgatacagc ttcgagatat tttgaaatcc gcattgcagg
901ttgaacagaa agaggactcc caaattcgtc agagcacagc ttatagctta catcagcctc
961agggaaacaa ggaacatggg accgagcgga acggcagcct ctacaagaag agtgacggga
1021tccgaaaagt gtggcagaaa aggaaatgtt cagttaaaaa tggttttctg accatatccc
1081atggtaccgc taaccggcct cctgcaaagc tcaacctgct aacctgccag gtgaagacca
1141accctgagga gaagaagtgc tttgacctta tttcacatga cagaacttac cactttcaag
1201ctgaagatga acaggaatgt caaatatgga tgtctgtgct gcaaaatagc aaagaagaag
1261ctttaaacaa tgcatttaag ggggatgaca atactggaga aaataacatc gtccaagaac
1321tgacaaagga gatcatctca gaagtgcaga ggatgacggg caatgacgtc tgctgtgact
1381gtggggcgcc agatcctaca tggctttcca ccaacctggg catcctgacc tgcatcgagt
1441gttccggaat ccaccgagag ctgggggttc attattccag gatgcagtcc ctgaccttag
1501atgtactggg aacatctgag ctgctgctcg ccaagaatat tgggaatgca ggctttaatg
1561agatcatgga atgttgccta ccagctgagg actcagtcaa acccaaccca ggcagcgaca
1621tgaatgcaag aaaggactac atcacagcca agtacatcga gaggagatac gcaaggaaga
1681agcacgcgga taacgcggcg aagcttcaca gtctttgcga ggccgtcaaa acgagagata
1741tttttggatt gctccaagct tatgctgatg gtgtggatct tacggaaaaa atcccactgg
1801ccaacggaca tgagccggat gaaacggccc tccaccttgc agtcagatcc gtggatcgaa
1861cctctcttca cattgtagac tttttagttc agaacagtgg gaacctggat aaacagacag
1921ggaaaggcag cacagccctg cactactgct gcctgaccga caatgccgag tgcctcaagt
1981tgctcctgcg ggggaaggcc tccatcgaga tagcaaacga gtcaggagag actccgctgg
2041acattgccaa gcgcctcaag cacgagcact gtgaggagct gctgacccaa gccttatctg
2101gaagatttaa ttctcacgtt cacgttgaat atgaatggcg actactccac gaagacctgg
2161atgaaagtga tgacgacatg gatgagaaat tgcagcccag tcccaaccgg cgggaagacc
2221ggcccatcag cttctaccag ctgggctcca accagcttca gtctaacgct gtatctttgg
2281ccagagatgc tgcaaacctt gccaaggaca agcagagggc tttcatgccc agcatcttgc
2341agaatgagac ttacggagcc ctcctgagtg gcagcccacc tcccgcccag cctgcagccc
2401ccagcaccac cagcgccccc ccgcttcctc cacggaatgt tggcaaagat cccctgaccc
2461ccacgccgcc cccacccgtt gccaagacgc ccagcgtaat ggaagccttg agccagccga
2521gcaagcctgc cccgcctggg atctcacaga tcaggccccc acctctgccc ccacagccgc
2581ccagccgcct cccgcagaag aagccTGCGC CGGGGGCTGA CAAGTCCACC CCACTGACCA
2641ACAAAGGCCA ACCGAGAGGA CCTGTGGATC TCTCTGCAAC GGAAGCTCTG GGTCCTCTGT
2701CCAATGCTAT GGTCCTGCAG CCCCCTGCAC CCATGCCTAG GAAGTCGCAG GCAACCAAGT
2761TGAAGCCTAA GCGGGTGAAA GCGCTCTATA ACTGTGTGGC TGACAACCCC GATGAGCTCA
2821CCTTCTCCGA GGGGGATGTG ATCATCGTGG ACGGGGAGGA GGACCAGGAG TGGTGGATTG
2881GCCACATTGA TGGAGATCCT GGTCGCAAAG GCGCATTCCC GGTGTCATTT GTGCACTTTA
2941TCGCTGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
3001CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
3061TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGACTT TAATCGCTTT ACTTAGAAAC
3121GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)