Transcript: Human XM_011512032.3

PREDICTED: Homo sapiens diacylglycerol kinase delta (DGKD), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DGKD (8527)
Length:
6188
CDS:
80..3550

Additional Resources:

NCBI RefSeq record:
XM_011512032.3
NBCI Gene record:
DGKD (8527)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146827 TGCACTTGGCGCTCACAGGT pXPR_003 AGG 557 16% 7 0.4163 DGKD DGKD 77257
2 BRDN0001147131 CGTCACCGAAGACTTCAGCG pXPR_003 AGG 1231 35% 12 0.1326 DGKD DGKD 77258
3 BRDN0001148084 CTCACGGCACACATTGCAGT pXPR_003 AGG 355 10% 5 -0.2002 DGKD DGKD 77259
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011512032.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000000674 CGCCTCGTGACCAAGTTTAAA pLKO.1 3257 CDS 100% 15.000 21.000 N DGKD n/a
2 TRCN0000231529 ATGGTTCACACATCGTGTAAA pLKO_005 722 CDS 100% 13.200 18.480 N DGKD n/a
3 TRCN0000231532 TAGAGTATTACACGGAGAAAT pLKO_005 2169 CDS 100% 13.200 18.480 N DGKD n/a
4 TRCN0000194834 CGAACGCTTTACTATGCCAAA pLKO.1 143 CDS 100% 4.050 5.670 N DGKD n/a
5 TRCN0000231531 CACCTCGGCTTACGGTTATTC pLKO_005 983 CDS 100% 13.200 10.560 N DGKD n/a
6 TRCN0000000671 CCAGTCTTGTTGATTGTAATT pLKO.1 4104 3UTR 100% 13.200 10.560 N DGKD n/a
7 TRCN0000196248 GCTCATCTTGTGTGCTGATAA pLKO.1 274 CDS 100% 13.200 10.560 N DGKD n/a
8 TRCN0000195008 CCAGAAACCCTAGAGTATTAC pLKO.1 2159 CDS 100% 13.200 9.240 N DGKD n/a
9 TRCN0000000672 GACAGCCTCAACCTTCATAAA pLKO.1 1076 CDS 100% 13.200 9.240 N DGKD n/a
10 TRCN0000231533 TCCAGTCTTGTTGATTGTAAT pLKO_005 4103 3UTR 100% 13.200 9.240 N DGKD n/a
11 TRCN0000195253 CATCATTCGATGACAAGATTC pLKO.1 2499 CDS 100% 10.800 7.560 N DGKD n/a
12 TRCN0000000675 GAAGATTGGATTGCAGCATTA pLKO.1 308 CDS 100% 10.800 7.560 N DGKD n/a
13 TRCN0000000673 AGATCATAGAACACACAGAAA pLKO.1 1770 CDS 100% 4.950 3.465 N DGKD n/a
14 TRCN0000231530 CACTGTTGGTCTTCGTCAATT pLKO_005 867 CDS 100% 13.200 7.920 N DGKD n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011512032.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.