Transcript: Human XM_011514036.3

PREDICTED: Homo sapiens 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HMGCS1 (3157)
Length:
3914
CDS:
1587..2558

Additional Resources:

NCBI RefSeq record:
XM_011514036.3
NBCI Gene record:
HMGCS1 (3157)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011514036.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218729 GATCTTTCACTCACCATATTG pLKO_005 1778 CDS 100% 13.200 18.480 N HMGCS1 n/a
2 TRCN0000412510 GATCTTTCACTCACCATATTG pLKO_005 1778 CDS 100% 13.200 18.480 N Hmgcs1 n/a
3 TRCN0000045843 CCCATCATTTGGTCAACTATA pLKO.1 2293 CDS 100% 13.200 10.560 N HMGCS1 n/a
4 TRCN0000045844 CGACAAATCAAAGTCTGTGAA pLKO.1 426 5UTR 100% 4.950 3.960 N HMGCS1 n/a
5 TRCN0000230558 AGGTACTAATCTCCAATTAAA pLKO_005 2899 3UTR 100% 15.000 10.500 N HMGCS1 n/a
6 TRCN0000230556 CATCATTTGGTCAACTATATT pLKO_005 2295 CDS 100% 15.000 10.500 N HMGCS1 n/a
7 TRCN0000230557 ACATAGCAACTGAGCATATTC pLKO_005 2455 CDS 100% 13.200 9.240 N HMGCS1 n/a
8 TRCN0000418756 CAGAGACAATCATCGACAAAT pLKO_005 413 5UTR 100% 13.200 9.240 N Hmgcs1 n/a
9 TRCN0000230555 TGTTGCCCTTGAGATCTATTT pLKO_005 189 5UTR 100% 13.200 9.240 N HMGCS1 n/a
10 TRCN0000045846 CCACAGGAAATGCTAGACCTA pLKO.1 1006 5UTR 100% 2.640 1.848 N HMGCS1 n/a
11 TRCN0000045845 CCTGATATGCTATCTGAATAT pLKO.1 1614 CDS 100% 13.200 7.920 N HMGCS1 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1497 5UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 1334 5UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1497 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011514036.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.