Transcript: Human XM_011515813.2

PREDICTED: Homo sapiens ring finger protein 32 (RNF32), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RNF32 (140545)
Length:
2399
CDS:
231..1280

Additional Resources:

NCBI RefSeq record:
XM_011515813.2
NBCI Gene record:
RNF32 (140545)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011515813.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000039412 CCTGTGCTCATACAACACCAA pLKO.1 899 CDS 100% 2.640 3.696 N Rnf32 n/a
2 TRCN0000034104 GCATGAGTTCTGGGTTAAGTA pLKO.1 1345 3UTR 100% 5.625 4.500 N RNF32 n/a
3 TRCN0000433205 GATCAGTGCTTGGCCATAAAT pLKO_005 945 CDS 100% 15.000 10.500 N RNF32 n/a
4 TRCN0000414159 GAGAGCTTGCTTACTTCTAAA pLKO_005 1528 3UTR 100% 13.200 9.240 N RNF32 n/a
5 TRCN0000434613 CATGATCTTCAACTTCGAAAT pLKO_005 309 CDS 100% 10.800 7.560 N RNF32 n/a
6 TRCN0000034108 GAGAGGATGTGTTGTTAGAAA pLKO.1 782 CDS 100% 5.625 3.938 N RNF32 n/a
7 TRCN0000034106 TGCTCATACAACACCAACATT pLKO.1 903 CDS 100% 5.625 3.938 N RNF32 n/a
8 TRCN0000034105 CCCAAACTAGAAGACTCAGAA pLKO.1 441 CDS 100% 4.950 3.465 N RNF32 n/a
9 TRCN0000226362 GCTCATACAACACCAACATAG pLKO_005 904 CDS 100% 10.800 7.560 N Rnf32 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011515813.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14386 pDONR223 100% 64.4% 54.7% None (many diffs) n/a
2 ccsbBroad304_14386 pLX_304 0% 64.4% 54.7% V5 (many diffs) n/a
3 TRCN0000473770 CTGTGGCTAACTTGGAGCGGCTAC pLX_317 62.4% 64.4% 54.7% V5 (many diffs) n/a
4 ccsbBroadEn_13201 pDONR223 100% 57.7% 55.7% None (many diffs) n/a
5 ccsbBroad304_13201 pLX_304 0% 57.7% 55.7% V5 (many diffs) n/a
6 TRCN0000469877 TTACACTTCTAATATACCATCCTT pLX_317 57.5% 57.7% 55.7% V5 (many diffs) n/a
7 TRCN0000487699 GAATAAAGAGTCCCGAAAGTGAGT pLX_317 34.3% 57.7% 55.7% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV