Transcript: Human XM_011518670.2

PREDICTED: Homo sapiens cilia and flagella associated protein 77 (CFAP77), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CFAP77 (389799)
Length:
1795
CDS:
230..1036

Additional Resources:

NCBI RefSeq record:
XM_011518670.2
NBCI Gene record:
CFAP77 (389799)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011518670.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000129682 CCGGAATTATATCGCAATGAA pLKO.1 580 CDS 100% 5.625 7.875 N CFAP77 n/a
2 TRCN0000130536 GACCCGGAATTATATCGCAAT pLKO.1 577 CDS 100% 4.050 5.670 N CFAP77 n/a
3 TRCN0000130249 CCCGGAATTATATCGCAATGA pLKO.1 579 CDS 100% 4.950 3.465 N CFAP77 n/a
4 TRCN0000129050 GAAAGCCATCAAACTGGAGAA pLKO.1 829 CDS 100% 4.050 2.835 N CFAP77 n/a
5 TRCN0000130127 CAAACTGGAGAAGAAGCAGAA pLKO.1 838 CDS 100% 4.050 2.430 N CFAP77 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011518670.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13662 pDONR223 100% 90.1% 83.2% None (many diffs) n/a
2 ccsbBroad304_13662 pLX_304 0% 90.1% 83.2% V5 (many diffs) n/a
3 ccsbBroadEn_14495 pDONR223 100% 89.9% 82.8% None (many diffs) n/a
4 ccsbBroad304_14495 pLX_304 0% 89.9% 82.8% V5 (many diffs) n/a
5 TRCN0000479918 ATTTTATCCCTTGATTGCTTAGGA pLX_317 38.6% 89.9% 82.8% V5 (many diffs) n/a
Download CSV