Transcript: Human XM_011521577.2

PREDICTED: Homo sapiens myocyte enhancer factor 2A (MEF2A), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MEF2A (4205)
Length:
5436
CDS:
190..1725

Additional Resources:

NCBI RefSeq record:
XM_011521577.2
NBCI Gene record:
MEF2A (4205)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521577.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005132 GCCTAGAAATATAGAGCATTA pLKO.1 2411 3UTR 100% 10.800 15.120 N MEF2A n/a
2 TRCN0000431440 TTGGACTACTTGTTTCGTAAA pLKO_005 2000 3UTR 100% 10.800 15.120 N MEF2A n/a
3 TRCN0000418879 AGCTCTAGTAGCTCCTATGAT pLKO_005 1579 CDS 100% 5.625 7.875 N MEF2A n/a
4 TRCN0000005136 GCTAGCACTGATATGGACAAA pLKO.1 361 CDS 100% 4.950 6.930 N MEF2A n/a
5 TRCN0000005133 CCAGACCCTGATACTTCATAT pLKO.1 484 CDS 100% 13.200 10.560 N MEF2A n/a
6 TRCN0000005135 GCAGTTATCTCAGGGTTCCAA pLKO.1 1353 CDS 100% 3.000 2.400 N MEF2A n/a
7 TRCN0000427703 AGCAGAACCAACTCGGATATT pLKO_005 421 CDS 100% 13.200 9.240 N MEF2A n/a
8 TRCN0000415441 CTCTAACAAACTGTTTCAATA pLKO_005 339 CDS 100% 13.200 9.240 N MEF2A n/a
9 TRCN0000422926 GTGAAATAGCACTCATCATTT pLKO_005 311 CDS 100% 13.200 9.240 N MEF2A n/a
10 TRCN0000432718 GCATCAAGTCCGAACCGATTT pLKO_005 1403 CDS 100% 10.800 7.560 N MEF2A n/a
11 TRCN0000005134 GCTTAGGCAAAGTCATGCCTA pLKO.1 938 CDS 100% 0.264 0.185 N MEF2A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521577.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06575 pDONR223 100% 97.5% 97.6% None (many diffs) n/a
2 ccsbBroad304_06575 pLX_304 0% 97.5% 97.6% V5 (many diffs) n/a
3 TRCN0000472816 CTCACACGAGAGGTCGTGTTGCCG pLX_317 9.8% 97.5% 97.6% V5 (many diffs) n/a
4 ccsbBroadEn_10962 pDONR223 100% 79.7% 74.9% None (many diffs) n/a
5 ccsbBroad304_10962 pLX_304 0% 79.7% 74.9% V5 (many diffs) n/a
6 TRCN0000479180 TCCACGCGCTGTGCATCGGTCAAG pLX_317 29.8% 79.7% 74.9% V5 (many diffs) n/a
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