Transcript: Human XM_011528483.1

PREDICTED: Homo sapiens ADP ribosylation factor related protein 1 (ARFRP1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ARFRP1 (10139)
Length:
1808
CDS:
216..821

Additional Resources:

NCBI RefSeq record:
XM_011528483.1
NBCI Gene record:
ARFRP1 (10139)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011528483.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000303688 ACGGCGTCATCTACGTCATTG pLKO_005 496 CDS 100% 10.800 15.120 N ARFRP1 n/a
2 TRCN0000047759 CCGATTTAACAAGAACTACAA pLKO.1 332 CDS 100% 4.950 6.930 N ARFRP1 n/a
3 TRCN0000310740 TGAGTCCAAGCAGGCGTTTGA pLKO_005 542 CDS 100% 4.950 6.930 N ARFRP1 n/a
4 TRCN0000380523 CACCACCGTGGGCCTAAACAT pLKO_005 377 CDS 100% 1.875 2.625 N ARFRP1 n/a
5 TRCN0000380613 GTCTTTGTGGGACAAGTATTA pLKO_005 464 CDS 100% 13.200 9.240 N ARFRP1 n/a
6 TRCN0000047761 CCTCTCAATCCCTGACATCAA pLKO.1 638 CDS 100% 4.950 3.465 N ARFRP1 n/a
7 TRCN0000310423 CCTCTCAATCCCTGACATCAA pLKO_005 638 CDS 100% 4.950 3.465 N ARFRP1 n/a
8 TRCN0000047758 CGAAGACAAACTTTCCTCTAT pLKO.1 953 3UTR 100% 4.950 3.465 N ARFRP1 n/a
9 TRCN0000299575 CGAAGACAAACTTTCCTCTAT pLKO_005 953 3UTR 100% 4.950 3.465 N ARFRP1 n/a
10 TRCN0000047762 CGGCTCATGTTCTGGGACTTA pLKO.1 423 CDS 100% 4.950 3.465 N ARFRP1 n/a
11 TRCN0000047760 GCAGTCTTTGTGGGACAAGTA pLKO.1 461 CDS 100% 4.950 3.465 N ARFRP1 n/a
12 TRCN0000379806 CAATGCTGGGAAGACGACCTT pLKO_005 293 CDS 100% 2.640 1.848 N ARFRP1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011528483.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07562 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_07562 pLX_304 0% 100% 100% V5 (not translated due to frame shift) n/a
3 TRCN0000476314 GAACCTTCATTTATCTTCGGGCAG pLX_317 46.5% 100% 100% V5 (not translated due to prior stop codon) n/a
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