Construct: ORF TRCN0000476314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009783.1_s317c1
- Derived from:
- ccsbBroadEn_07562
- DNA Barcode:
- GAACCTTCATTTATCTTCGGGCAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARFRP1 (10139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267547.3 | 100% | 100% | |
| 2 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267548.2 | 100% | 100% | |
| 3 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_003224.6 | 100% | 100% | |
| 4 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | XM_011528482.3 | 100% | 100% | |
| 5 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | XM_011528483.1 | 100% | 100% | |
| 6 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | XM_017027576.1 | 100% | 100% | |
| 7 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001134758.4 | 85.9% | 86% | 519_519delGins85 |
| 8 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267549.3 | 85.9% | 86% | 519_519delGins85 |
| 9 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267546.3 | 76.6% | 76.6% | 0_1ins141 |
| 10 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267544.3 | 71.6% | 56.5% | 345_346insGA;432_433ins169 |
| 11 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NM_001267545.2 | 60.1% | 57.2% | 345_346ins71;363_364ins169 |
| 12 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | XR_001754110.1 | 33% | 1_215del;560_561insGAGAA;814_1803del | |
| 13 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NR_051954.3 | 23.9% | 1_111del;456_457insGA;713_2511del | |
| 14 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NR_051955.3 | 23.8% | 1_116del;461_462insGA;718_2516del | |
| 15 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NR_051957.3 | 23.7% | 1_111del;456_457insGAGAA;710_2508del | |
| 16 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NR_051956.3 | 23.7% | 1_116del;461_462insGAGAA;715_2513del | |
| 17 | human | 10139 | ARFRP1 | ADP ribosylation factor rel... | NR_051958.3 | 21.1% | 1_111del;456_457ins71;644_2442del | |
| 18 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | NM_001165991.1 | 88.5% | 97% | (many diffs) |
| 19 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | NM_001165992.1 | 88.5% | 97% | (many diffs) |
| 20 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | NM_029702.4 | 88.5% | 97% | (many diffs) |
| 21 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | XM_011239965.2 | 88.5% | 97% | (many diffs) |
| 22 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | NM_001165995.1 | 66.8% | 73.6% | (many diffs) |
| 23 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | XR_867137.2 | 29.6% | (many diffs) | |
| 24 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | XR_867138.2 | 26.7% | (many diffs) | |
| 25 | mouse | 76688 | Arfrp1 | ADP-ribosylation factor rel... | NR_028585.1 | 18.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 672
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtacacgctg ctgtcgggct tgtacaagta catgtttcag aaggacgagt 121 actgcatcct gatcctgggc ctggacaatg ctgggaagac gaccttcctg gagcagtcga 181 aaacccgatt taacaagaac tacaagggga tgagtctatc caaaatcacc accaccgtgg 241 gcctaaacat cggcactgtg gatgtgggaa aggctcggct catgttctgg gacttaggag 301 ggcaggaaga gctgcagtct ttgtgggaca agtattatgc ggagtgtcac ggcgtcatct 361 acgtcattga ctccaccgac gaggagaggc tGGCTGAGTC CAAGCAGGCG TTTGAGAAGG 421 TGGTGACCAG CGAGGCGCTG TGCGGTGTCC CCGTCTTGGT GCTGGCCAAC AAGCAGGATG 481 TGGAGACGTG CCTCTCAATC CCTGACATCA AGACGGCCTT CAGCGACTGC ACCAGCAAGA 541 TCGGCAGGCG AGATTGCCTG ACCCAGGCCT GCTCGGCCCT CACAGGCAAA GGGGTGCGCG 601 AGGGCATCGA GTGGATGGTG AAGTGTGTCG TGCGGAATGT GCACCGGCCG CCGCGGCAGA 661 GGGACATCAC GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 721 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 781 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA ACCTTCATTT ATCTTCGGGC 841 AGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt