Transcript: Human XM_011528900.2

PREDICTED: Homo sapiens ZFP64 zinc finger protein (ZFP64), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZFP64 (55734)
Length:
3123
CDS:
684..2255

Additional Resources:

NCBI RefSeq record:
XM_011528900.2
NBCI Gene record:
ZFP64 (55734)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011528900.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232917 CATTACCAGTTCGGGTATTAC pLKO_005 2018 CDS 100% 13.200 18.480 N ZFP64 n/a
2 TRCN0000232916 GAGTTTCCACTGCGATATATG pLKO_005 1478 CDS 100% 13.200 18.480 N ZFP64 n/a
3 TRCN0000232915 TATCAAGCACAGCGGGAATAA pLKO_005 1130 CDS 100% 13.200 18.480 N ZFP64 n/a
4 TRCN0000232914 GCTGACTCCCGACATCCATAT pLKO_005 165 5UTR 100% 10.800 15.120 N ZFP64 n/a
5 TRCN0000232918 CTCTGAATCGTCGCATTATAA pLKO_005 2748 3UTR 100% 15.000 12.000 N ZFP64 n/a
6 TRCN0000107447 CCGCCCTTTCAAGTGCAACTA pLKO.1 1313 CDS 100% 4.950 3.960 N ZFP64 n/a
7 TRCN0000107446 CTGGCAATCAAACTTTCATTA pLKO.1 2002 CDS 100% 13.200 9.240 N ZFP64 n/a
8 TRCN0000107445 CGTGGTTTGCTCTTAATGTTT pLKO.1 2631 3UTR 100% 5.625 3.938 N ZFP64 n/a
9 TRCN0000107448 GCAATCAAACTTTCATTACCA pLKO.1 2005 CDS 100% 3.000 2.100 N ZFP64 n/a
10 TRCN0000107449 TGCCAATTCAAGACTGCTTAT pLKO.1 660 5UTR 100% 10.800 6.480 N ZFP64 n/a
11 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 2552 3UTR 100% 4.950 2.475 Y LOC387873 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2589 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2589 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011528900.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08573 pDONR223 100% 76.6% 76.6% None (many diffs) n/a
2 ccsbBroad304_08573 pLX_304 0% 76.6% 76.6% V5 (many diffs) n/a
3 TRCN0000480194 GTCTGGGTACTACCATCCTTCCCT pLX_317 19.3% 76.6% 76.6% V5 (many diffs) n/a
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