Transcript: Human XM_011533766.2

PREDICTED: Homo sapiens Rho guanine nucleotide exchange factor 3 (ARHGEF3), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ARHGEF3 (50650)
Length:
3893
CDS:
459..2042

Additional Resources:

NCBI RefSeq record:
XM_011533766.2
NBCI Gene record:
ARHGEF3 (50650)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011533766.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412805 CTATTACCCTGTGTTGTATTT pLKO_005 2240 3UTR 100% 13.200 18.480 N ARHGEF3 n/a
2 TRCN0000047543 GCCTAGTAATAAACGGGTCAA pLKO.1 560 CDS 100% 4.050 5.670 N ARHGEF3 n/a
3 TRCN0000430081 GAATCTGAATGCCGCTATTAT pLKO_005 1380 CDS 100% 15.000 10.500 N ARHGEF3 n/a
4 TRCN0000437024 CCCTCTGCTTCTCCGAGAAAT pLKO_005 1259 CDS 100% 13.200 9.240 N ARHGEF3 n/a
5 TRCN0000047547 CCCATGCTGAAACTCTCCATA pLKO.1 912 CDS 100% 4.950 3.465 N ARHGEF3 n/a
6 TRCN0000047546 CGCAAACTAGATCTCTGGAAT pLKO.1 1203 CDS 100% 4.950 3.465 N ARHGEF3 n/a
7 TRCN0000047545 GCGATCTTTGAGCTTTCCCAA pLKO.1 837 CDS 100% 2.640 1.848 N ARHGEF3 n/a
8 TRCN0000047544 CGGCTTCTTTACTTGGAAGAA pLKO.1 1407 CDS 100% 0.495 0.347 N ARHGEF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011533766.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03149 pDONR223 100% 95.7% 91.9% None (many diffs) n/a
2 ccsbBroad304_03149 pLX_304 0% 95.7% 91.9% V5 (many diffs) n/a
3 TRCN0000476018 ACGGCTAGCTGTTATCGGGTACTC pLX_317 24.4% 95.7% 91.9% V5 (many diffs) n/a
4 ccsbBroadEn_11925 pDONR223 100% 61.1% 60.9% None 1_612delinsA;614_615insAA;1006T>G n/a
5 ccsbBroad304_11925 pLX_304 0% 61.1% 60.9% V5 1_612delinsA;614_615insAA;1006T>G n/a
6 TRCN0000474076 TTTTGCCACTACTCGGCCACGTTG pLX_317 39.2% 61.1% 60.9% V5 1_612delinsA;614_615insAA;1006T>G n/a
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