Transcript: Human XM_011535290.1

PREDICTED: Homo sapiens sciellin (SCEL), transcript variant X14, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SCEL (8796)
Length:
2995
CDS:
117..2006

Additional Resources:

NCBI RefSeq record:
XM_011535290.1
NBCI Gene record:
SCEL (8796)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011535290.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000163623 GTGGTGCTCAACCGACATAAT pLKO.1 297 CDS 100% 13.200 18.480 N SCEL n/a
2 TRCN0000161906 GCCAATACCTTGGATAACCAA pLKO.1 444 CDS 100% 3.000 4.200 N SCEL n/a
3 TRCN0000164519 CCGGACAGATAAAGATGGCAA pLKO.1 1010 CDS 100% 2.640 3.696 N SCEL n/a
4 TRCN0000158492 CCTTGAAAGTCTCATCTATAT pLKO.1 983 CDS 100% 13.200 9.240 N SCEL n/a
5 TRCN0000159590 GAAGATCAACTCTTGTACAAA pLKO.1 2104 3UTR 100% 5.625 3.938 N SCEL n/a
6 TRCN0000158464 CATTGAAGATCAACTCTTGTA pLKO.1 2100 3UTR 100% 4.950 3.465 N SCEL n/a
7 TRCN0000159245 GCACAAGGAAATCAAGATGAA pLKO.1 2012 3UTR 100% 4.950 3.465 N SCEL n/a
8 TRCN0000158951 GCTACTGAAGTAAATCCCAAA pLKO.1 1173 CDS 100% 4.050 2.835 N SCEL n/a
9 TRCN0000160772 CCACCTAAACCAGGTGTATAT pLKO.1 753 CDS 100% 1.320 0.924 N SCEL n/a
10 TRCN0000159993 CACAAGGAAATCAAGATGAAA pLKO.1 2013 3UTR 100% 5.625 3.375 N SCEL n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011535290.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07305 pDONR223 100% 88.3% 88% None (many diffs) n/a
2 ccsbBroad304_07305 pLX_304 0% 88.3% 88% V5 (many diffs) n/a
3 TRCN0000478482 GACCCAGAACGGCAGAGCTCAGAC pLX_317 15.2% 88.3% 88% V5 (many diffs) n/a
4 ccsbBroadEn_15644 pDONR223 0% 44.8% 44.6% None (many diffs) n/a
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