Transcript: Human XM_011538532.3

PREDICTED: Homo sapiens adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2 (APPL2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
APPL2 (55198)
Length:
5674
CDS:
2789..4672

Additional Resources:

NCBI RefSeq record:
XM_011538532.3
NBCI Gene record:
APPL2 (55198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011538532.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000420727 AGAAGCACTGGCTCAATTAAT pLKO_005 4543 CDS 100% 15.000 12.000 N APPL2 n/a
2 TRCN0000413949 AGCACTTTAAAGGATCTATTT pLKO_005 3038 CDS 100% 13.200 10.560 N APPL2 n/a
3 TRCN0000153927 CTCAAGTATCAAGGGCCAATT pLKO.1 4329 CDS 100% 10.800 8.640 N APPL2 n/a
4 TRCN0000154282 GCTGACAATCGTTTGTGGAAT pLKO.1 4773 3UTR 100% 4.950 3.960 N APPL2 n/a
5 TRCN0000435347 ATCTCATAATTGGACCTAATA pLKO_005 5147 3UTR 100% 13.200 9.240 N APPL2 n/a
6 TRCN0000153500 CCCACCCATCTACAATGTTAT pLKO.1 4855 3UTR 100% 13.200 9.240 N APPL2 n/a
7 TRCN0000150613 GAGAAGAATCTCTGAGTACAT pLKO.1 4437 CDS 100% 4.950 3.465 N APPL2 n/a
8 TRCN0000153746 CTGGAGAAGAATCTCTGAGTA pLKO.1 4434 CDS 100% 4.950 2.970 N APPL2 n/a
9 TRCN0000152600 GCAGACATGGTTCAAAGCATT pLKO.1 3365 CDS 100% 4.950 2.970 N APPL2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011538532.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08489 pDONR223 100% 92.4% 92.5% None (many diffs) n/a
2 ccsbBroad304_08489 pLX_304 0% 92.4% 92.5% V5 (many diffs) n/a
3 TRCN0000481225 AGTAGTAGCAGAAGGTTCGGATTC pLX_317 18.7% 92.4% 92.5% V5 (many diffs) n/a
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