Construct: ORF TRCN0000481225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011612.1_s317c1
- Derived from:
- ccsbBroadEn_08489
- DNA Barcode:
- AGTAGTAGCAGAAGGTTCGGATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APPL2 (55198)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | NM_018171.5 | 99.7% | 99.8% | (many diffs) |
| 2 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | NM_001251904.1 | 98.9% | 98.9% | (many diffs) |
| 3 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_017019551.2 | 97.5% | 97.4% | (many diffs) |
| 4 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_011538530.3 | 96.7% | 96.5% | (many diffs) |
| 5 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | NM_001251905.1 | 93.3% | 93.3% | (many diffs) |
| 6 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_017019552.2 | 93.3% | 93.3% | (many diffs) |
| 7 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_017019553.2 | 93.3% | 93.3% | (many diffs) |
| 8 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_017019554.1 | 92.7% | 92.7% | (many diffs) |
| 9 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_011538531.3 | 92.4% | 92.5% | (many diffs) |
| 10 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_011538532.3 | 92.4% | 92.5% | (many diffs) |
| 11 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XM_006719472.1 | 91.8% | 91.9% | (many diffs) |
| 12 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XR_001748796.1 | 62.6% | (many diffs) | |
| 13 | human | 55198 | APPL2 | adaptor protein, phosphotyr... | XR_001748795.1 | 62.2% | (many diffs) | |
| 14 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | NM_145220.2 | 87.6% | 92.3% | (many diffs) |
| 15 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_006513508.3 | 82.6% | 84.5% | (many diffs) |
| 16 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_006513509.3 | 81.9% | 86.8% | (many diffs) |
| 17 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_006513510.2 | 80.4% | 84.4% | (many diffs) |
| 18 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_011243404.1 | 80.4% | 84.4% | (many diffs) |
| 19 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_017313895.1 | 74.7% | 79% | (many diffs) |
| 20 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XM_006513511.3 | 72.9% | 76.3% | (many diffs) |
| 21 | mouse | 216190 | Appl2 | adaptor protein, phosphotyr... | XR_380384.3 | 54.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2058
- ORF length:
- 1992
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgccgtggac aagctcctgc tagaggaggc gttgcaggac agcccccaga 121 ctcgctcttt actgagcgtg tttgaagaag atgctggcac cctcacagac tataccaacc 181 agctgctcca ggcaatgcag cgcgtctatg gagcccagaa tgagatgtgc ctggccacac 241 aacagctttc taagcaactg ctggcatatg aaaaacagaa ctttgctctt ggcaaaggtg 301 atgaagaagt aatttcaaca ctccactatt tttccaaagt ggtggatgag cttaatcttc 361 tccatacaga gctggctaaa cagttggcag acacaatggt tctacctatc atacaattcc 421 gagaaaagga tctcacagaa gtaagcactt taaaggatct atttggactc gctagcaatg 481 agcatgacct ctcaatggca aaatacagca ggctgcctaa gaaaaaggag aatgagaagg 541 tgaagaccga agtcggaaaa gaggtggccg cggcccggcg gaagcagcat ctctcctccc 601 ttcagtacta ctgtgccctc aacgcgctgc agtacagaaa gcaaatggcc atgatggagc 661 ccatgatagg ctttgcccat ggacagatta acttttttaa gaagggagca gagatgtttt 721 ccaaacgtat ggacagcttt ttatcctccg ttgcagacat ggttcaaagc attcaggtag 781 aactggaagc cgaggcggaa aagatgcggg tgtcccagca agaattactt tctgttgatg 841 aatctgttta cactccagac tctgatgtgg ccgcaccaca gatcaacagg aacctcatcc 901 agaaggctgg ttaccttaat cttagaaaca aaacagggct ggtcaccacc acctgggaga 961 ggctttattt cttcacccaa ggcgggaatc tcatgtgtca gcccagggga gccgtggctg 1021 gaggtttgat ccaggacctg gacaactgct cagtgatggc cgtggattgc gaagaccggc 1081 gctactgctt ccagatcacc acgcccaatg gaaaatcggg aataatcctc caggctgaga 1141 gcagaaagga aaatgaagag tggatatgtg caataaacaa catctccaga cagatctacc 1201 tgaccgacaa ccctgaggca gtcgcgatca agttgaatca gaccgctctg caagcagtga 1261 ctcccattac aagttttgga aaaaaacaag aaagctcatg ccccagccag aacctgaaaa 1321 attcagagat ggaaaatgaa aatgacaaga ttgttcccaa agtaacagcc agtctacctg 1381 aagcagagga gctgatcgcg cctggaacgc cgattcaatt cgatattgtg cttcctgcta 1441 cagaattcct tgatcagaac agagggagca ggcgtaccaa cccttttggt gaaactgagg 1501 atgaatcatt tccagaagca gaagattctc ttttgcagca gatgtttata gttcggtttt 1561 tgggatcaat ggcagttaaa acagacagca ctactgaagt gatttatgaa gcgatgagac 1621 aagtattggc tgctcgggct attcataaca tcttccgcat gacagaatcc catctgatgg 1681 tcaccagtca aTCTTTGAGG TTGATAGATC CACAGACTCA AGTATCAAGG GCCAATTTTG 1741 AACTTACCAG TGTCACACAA TTTGCTGCTC ATCAAGAAAA CAAGAGACTG GTTGGTTTTG 1801 TCATCCGTGT TCCTGAATCC ACTGGAGAAG AATCTCTGAG TACATACATT TTTGAAAGCA 1861 ACTCAGAAGG CGAAAAGATA TGTTATGCTA TTAATTTGGG AAAAGAAATT ATTGAGGTTC 1921 AGAAGGATCC AGAAGCACTG GCTCAATTAA TGCTGTCCAT ACCACTAACC AATGATGGAA 1981 AATATGTACT GTTAAACGAT CAACCAGATG ACGATGATGG AAATCCAAAT GAACATAGAG 2041 GCGCAGAATC CGAAGCATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2101 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2161 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGTAGTA GCAGAAGGTT 2221 CGGATTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt