Transcript: Human XM_011544257.1

PREDICTED: Homo sapiens SET and MYND domain containing 3 (SMYD3), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SMYD3 (64754)
Length:
1097
CDS:
100..876

Additional Resources:

NCBI RefSeq record:
XM_011544257.1
NBCI Gene record:
SMYD3 (64754)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544257.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000308170 GAAGTTGGTGTTGGCCTATAT pLKO_005 163 CDS 100% 13.200 10.560 N SMYD3 n/a
2 TRCN0000123290 GCTTCCCGATATCAACATCTA pLKO.1 546 CDS 100% 4.950 3.960 N SMYD3 n/a
3 TRCN0000289580 GCTTCCCGATATCAACATCTA pLKO_005 546 CDS 100% 4.950 3.960 N SMYD3 n/a
4 TRCN0000308169 GAACGCAGTCAGAGGGAAATA pLKO_005 879 3UTR 100% 13.200 9.240 N SMYD3 n/a
5 TRCN0000123292 AGCCTGATTGAAGATTTGATT pLKO.1 814 CDS 100% 5.625 3.938 N SMYD3 n/a
6 TRCN0000123293 CAGCCTGATTGAAGATTTGAT pLKO.1 813 CDS 100% 5.625 3.938 N SMYD3 n/a
7 TRCN0000289526 CAGCCTGATTGAAGATTTGAT pLKO_005 813 CDS 100% 5.625 3.938 N SMYD3 n/a
8 TRCN0000123291 CAACTCTTTCACCATCTGTAA pLKO.1 129 CDS 100% 4.950 3.465 N SMYD3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544257.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12481 pDONR223 100% 92.6% 92.6% None 1_57del n/a
2 ccsbBroad304_12481 pLX_304 0% 92.6% 92.6% V5 1_57del n/a
3 TRCN0000479013 CTTTCTAGTAATTGAAGCAAACCG pLX_317 48.9% 92.6% 92.6% V5 1_57del n/a
4 ccsbBroadEn_08862 pDONR223 100% 59.9% 59.1% None (many diffs) n/a
5 ccsbBroad304_08862 pLX_304 0% 59.9% 59.1% V5 (many diffs) n/a
6 TRCN0000473342 TGCATATGGCCGTAGACGGCTAGT pLX_317 38.4% 59.9% 59.1% V5 (many diffs) n/a
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