Construct: ORF TRCN0000473342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013482.1_s317c1
- Derived from:
- ccsbBroadEn_08862
- DNA Barcode:
- TGCATATGGCCGTAGACGGCTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMYD3 (64754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64754 | SMYD3 | SET and MYND domain contain... | NM_001167740.2 | 99.7% | 99.5% | 39G>C;419A>G;933A>G |
| 2 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544253.1 | 91.1% | 90.8% | (many diffs) |
| 3 | human | 64754 | SMYD3 | SET and MYND domain contain... | NM_022743.2 | 86% | 85.9% | 0_1ins177;242A>G;756A>G |
| 4 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449136.1 | 86% | 85.9% | 0_1ins177;242A>G;756A>G |
| 5 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449137.1 | 86% | 85.9% | 0_1ins177;242A>G;756A>G |
| 6 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544254.2 | 63% | 57.4% | (many diffs) |
| 7 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544257.1 | 59.9% | 59.1% | (many diffs) |
| 8 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544258.2 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 9 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544259.3 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 10 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544260.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 11 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544261.3 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 12 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_017002094.2 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 13 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449141.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 14 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449148.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 15 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449149.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 16 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449156.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 17 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449162.1 | 55.7% | 55.8% | 0_1ins567;366A>G |
| 18 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449166.1 | 54.7% | 53.2% | (many diffs) |
| 19 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | NM_027188.3 | 87.3% | 93.9% | (many diffs) |
| 20 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_011238864.2 | 77.2% | 80.1% | (many diffs) |
| 21 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_011238865.2 | 52.8% | 55.8% | (many diffs) |
| 22 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_006496982.3 | 49.2% | 53% | (many diffs) |
| 23 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_017312211.1 | 49.2% | 53% | (many diffs) |
| 24 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_017312213.1 | 49.2% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccgctgaag gtggaaaagt tcgcaaccgc caacagggga aacgggctgc 121 gcgccgtgac cccgctgcgc cccggagagc tactcttccg ctcggatccc ttggcgtaca 181 cggtgtgcaa ggggagtcgt ggcgtcgtct gcgaccgctg ccttctcggg aaggaaaagc 241 tgatgcgatg ctctcagtgc cgcgtcgcca aatactgtag tgctaagtgt cagaaaaaag 301 cttggccaga ccacaagcgg gaatgcaaat gccttaaaag ctgcaaaccc agatatcctc 361 cagactccgt tcgacttctt ggcagagttg tcttcaaact tatggatgga gcaccttcag 421 aatcagagaa gctttactca ttttatgatc tggagtcaaa tattaacaaa ctgactgaag 481 ataggaaaga gggcctcagg caactcgtaa tgacatttca acatttcatg agagaagaaa 541 tacaggatgc ctctcagctg ccacctgcct ttgacctttt tgaagccttt gcaaaagtga 601 tctgcaactc tttcaccatc tgtaatgcgg agatgcagga agttggtgtt ggcctatatc 661 ccagtatctc tttgctcaat cacagctgtg accccaactg ttcgattgtg ttcaatgggc 721 cccacctctt actgcgagca gtccgagaca tcgaggtggg agaggagctc accatctgct 781 acctggatat gctgatgacc agtgaggagc gccggaagca gctgagggac cagtactgct 841 ttgaatgtga ctgtttccGT TGCCAAACCC AGGACAAGGA TGCTGATATG CTAACTGGTG 901 ATGAGCAAGT ATGGAAGGAA GTTCAAGAAT CCCTGAAAAA AATTGAAGAA CTGAAGGCAC 961 ACTGGAAGTG GGAGCAGGTT CTGGCCATGT GCCAGGCGAT CATAAGCAGC AATTCTGAAC 1021 GGCTTCCCGA TATCAACATC TACCAGCTGA AGGTGCTCGA CTGCGCCATG GATGCCTGCA 1081 TCAACCTCGG CCTGTTGGAG GAAGCCTTGT TCTATGGTAC TCGGACCATG GAGCCATACA 1141 GGATTTTTTT CCCAGGAAGC CATCCCGTCA GAGGGGTTCA AGTGATGAAA GTTGGCAAAC 1201 TGCAGCTACA TCAAGGCATG TTTCCCCAAG CAATGAAGAA TCTGAGACTG GCTTTTGATA 1261 TTATGAGAGT GACACATGGC AGAGAACACA GCCTGATTGA AGATTTGATT CTACTTTTAG 1321 AAGAATGCGA CGCCAACATC AGAGCATCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGCAT 1501 ATGGCCGTAG ACGGCTAGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt