Construct: ORF TRCN0000479013

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004234.1_s317c1
Derived from:
ccsbBroadEn_12481
DNA Barcode:
CTTTCTAGTAATTGAAGCAAACCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SMYD3 (64754)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479013
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64754 SMYD3 SET and MYND domain contain... XM_011544258.2 100% 100%
2 human 64754 SMYD3 SET and MYND domain contain... XM_011544259.3 100% 100%
3 human 64754 SMYD3 SET and MYND domain contain... XM_011544260.1 100% 100%
4 human 64754 SMYD3 SET and MYND domain contain... XM_011544261.3 100% 100%
5 human 64754 SMYD3 SET and MYND domain contain... XM_017002094.2 100% 100%
6 human 64754 SMYD3 SET and MYND domain contain... XM_024449141.1 100% 100%
7 human 64754 SMYD3 SET and MYND domain contain... XM_024449148.1 100% 100%
8 human 64754 SMYD3 SET and MYND domain contain... XM_024449149.1 100% 100%
9 human 64754 SMYD3 SET and MYND domain contain... XM_024449156.1 100% 100%
10 human 64754 SMYD3 SET and MYND domain contain... XM_024449162.1 100% 100%
11 human 64754 SMYD3 SET and MYND domain contain... XM_024449166.1 96.9% 95% (many diffs)
12 human 64754 SMYD3 SET and MYND domain contain... XM_011544257.1 92.6% 92.6% 1_57del
13 human 64754 SMYD3 SET and MYND domain contain... XM_011544254.2 83.5% 83.5% 1_141del
14 human 64754 SMYD3 SET and MYND domain contain... NM_022743.2 64.7% 64.7% 1_390del
15 human 64754 SMYD3 SET and MYND domain contain... XM_024449136.1 64.7% 64.7% 1_390del
16 human 64754 SMYD3 SET and MYND domain contain... XM_024449137.1 64.7% 64.7% 1_390del
17 human 64754 SMYD3 SET and MYND domain contain... NM_001167740.2 55.8% 55.8% 1_567del
18 human 64754 SMYD3 SET and MYND domain contain... XM_011544253.1 47.1% 47.1% 1_567del;700_701ins111
19 mouse 69726 Smyd3 SET and MYND domain contain... XM_006496982.3 88% 94.9% (many diffs)
20 mouse 69726 Smyd3 SET and MYND domain contain... XM_017312211.1 88% 94.9% (many diffs)
21 mouse 69726 Smyd3 SET and MYND domain contain... XM_017312213.1 88% 94.9% (many diffs)
22 mouse 69726 Smyd3 SET and MYND domain contain... XM_011238865.2 80.8% 87.3% (many diffs)
23 mouse 69726 Smyd3 SET and MYND domain contain... NM_027188.3 49.1% 53% (many diffs)
24 mouse 69726 Smyd3 SET and MYND domain contain... XM_011238864.2 39.1% 39.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
783
ORF length:
717
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61tcggcatgca ggaagttggt gttggcctat atcccagtat ctctttgctc aatcacagct
121gtgaccccaa ctgttcgatt gtgttcaatg ggccccacct cttactgcga gcagtccgag
181acatcgaggt gggagaggag ctcaccatct gctacctgga tatgctgatg accagtgagg
241agcgccggaa gcagctgagg gaccagtact gctttgaatg tgactgtttc cgttgccaaa
301cccaggacaa ggatgctgat atgctaactg gtgatgagca agtatggaag gaagttcaag
361aatccctgaa aaaaattgaa gaactgaagg cacactggaa gtgggagcag gttctggcca
421tgtgccaggc aaTCATAAGC AGCAATTCTG AACGGCTTCC CGATATCAAC ATCTACCAGC
481TGAAGGTGCT CGACTGCGCC ATGGATGCCT GCATCAACCT CGGCCTGTTG GAGGAAGCCT
541TGTTCTATGG TACTCGGACC ATGGAGCCAT ACAGGATTTT TTTCCCAGGA AGCCATCCCG
601TCAGAGGGGT TCAAGTGATG AAAGTTGGCA AACTGCAGCT ACATCAAGGC ATGTTTCCCC
661AAGCAATGAA GAATCTGAGA CTGGCTTTTG ATATTATGAG AGTGACACAT GGCAGAGAAC
721ACAGCCTGAT TGAAGATTTG ATTCTACTTT TAGAAGAATG CGACGCCAAC ATCAGAGCAT
781CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
841TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
901GGCTTTATAT ATCTTGTGGA AAGGACGACT TTCTAGTAAT TGAAGCAAAC CGACGCGTTA
961AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)