Construct: ORF TRCN0000479013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004234.1_s317c1
- Derived from:
- ccsbBroadEn_12481
- DNA Barcode:
- CTTTCTAGTAATTGAAGCAAACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMYD3 (64754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544258.2 | 100% | 100% | |
| 2 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544259.3 | 100% | 100% | |
| 3 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544260.1 | 100% | 100% | |
| 4 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544261.3 | 100% | 100% | |
| 5 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_017002094.2 | 100% | 100% | |
| 6 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449141.1 | 100% | 100% | |
| 7 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449148.1 | 100% | 100% | |
| 8 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449149.1 | 100% | 100% | |
| 9 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449156.1 | 100% | 100% | |
| 10 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449162.1 | 100% | 100% | |
| 11 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449166.1 | 96.9% | 95% | (many diffs) |
| 12 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544257.1 | 92.6% | 92.6% | 1_57del |
| 13 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544254.2 | 83.5% | 83.5% | 1_141del |
| 14 | human | 64754 | SMYD3 | SET and MYND domain contain... | NM_022743.2 | 64.7% | 64.7% | 1_390del |
| 15 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449136.1 | 64.7% | 64.7% | 1_390del |
| 16 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_024449137.1 | 64.7% | 64.7% | 1_390del |
| 17 | human | 64754 | SMYD3 | SET and MYND domain contain... | NM_001167740.2 | 55.8% | 55.8% | 1_567del |
| 18 | human | 64754 | SMYD3 | SET and MYND domain contain... | XM_011544253.1 | 47.1% | 47.1% | 1_567del;700_701ins111 |
| 19 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_006496982.3 | 88% | 94.9% | (many diffs) |
| 20 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_017312211.1 | 88% | 94.9% | (many diffs) |
| 21 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_017312213.1 | 88% | 94.9% | (many diffs) |
| 22 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_011238865.2 | 80.8% | 87.3% | (many diffs) |
| 23 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | NM_027188.3 | 49.1% | 53% | (many diffs) |
| 24 | mouse | 69726 | Smyd3 | SET and MYND domain contain... | XM_011238864.2 | 39.1% | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcatgca ggaagttggt gttggcctat atcccagtat ctctttgctc aatcacagct 121 gtgaccccaa ctgttcgatt gtgttcaatg ggccccacct cttactgcga gcagtccgag 181 acatcgaggt gggagaggag ctcaccatct gctacctgga tatgctgatg accagtgagg 241 agcgccggaa gcagctgagg gaccagtact gctttgaatg tgactgtttc cgttgccaaa 301 cccaggacaa ggatgctgat atgctaactg gtgatgagca agtatggaag gaagttcaag 361 aatccctgaa aaaaattgaa gaactgaagg cacactggaa gtgggagcag gttctggcca 421 tgtgccaggc aaTCATAAGC AGCAATTCTG AACGGCTTCC CGATATCAAC ATCTACCAGC 481 TGAAGGTGCT CGACTGCGCC ATGGATGCCT GCATCAACCT CGGCCTGTTG GAGGAAGCCT 541 TGTTCTATGG TACTCGGACC ATGGAGCCAT ACAGGATTTT TTTCCCAGGA AGCCATCCCG 601 TCAGAGGGGT TCAAGTGATG AAAGTTGGCA AACTGCAGCT ACATCAAGGC ATGTTTCCCC 661 AAGCAATGAA GAATCTGAGA CTGGCTTTTG ATATTATGAG AGTGACACAT GGCAGAGAAC 721 ACAGCCTGAT TGAAGATTTG ATTCTACTTT TAGAAGAATG CGACGCCAAC ATCAGAGCAT 781 CCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGACT TTCTAGTAAT TGAAGCAAAC CGACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt