Transcript: Human XM_017001420.2

PREDICTED: Homo sapiens pleckstrin homology domain containing O1 (PLEKHO1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PLEKHO1 (51177)
Length:
2138
CDS:
883..1596

Additional Resources:

NCBI RefSeq record:
XM_017001420.2
NBCI Gene record:
PLEKHO1 (51177)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000165365 GAGAGACCTGTACAGACAGAT pLKO.1 1503 CDS 100% 4.950 6.930 N PLEKHO1 n/a
2 TRCN0000165364 GAGAGGCATCATCGAATTGGA pLKO.1 1445 CDS 100% 3.000 4.200 N PLEKHO1 n/a
3 TRCN0000292422 GAGAGGCATCATCGAATTGGA pLKO_005 1445 CDS 100% 3.000 4.200 N PLEKHO1 n/a
4 TRCN0000202420 GAAATTCTGCGGGAAAGGGAT pLKO.1 401 5UTR 100% 2.640 3.696 N Plekho1 n/a
5 TRCN0000164405 CAAGAACCGTATCTTGGATGA pLKO.1 768 5UTR 100% 0.405 0.567 N PLEKHO1 n/a
6 TRCN0000165626 GCCAAGAACCGTATCTTGGAT pLKO.1 766 5UTR 100% 0.300 0.420 N PLEKHO1 n/a
7 TRCN0000292421 GCCAAGAACCGTATCTTGGAT pLKO_005 766 5UTR 100% 0.300 0.420 N PLEKHO1 n/a
8 TRCN0000165294 GCAAGTTTACTCTTGCCCACT pLKO.1 592 5UTR 100% 0.216 0.173 N PLEKHO1 n/a
9 TRCN0000339908 TCCGGAAATTCTGCGGGAAAG pLKO_005 397 5UTR 100% 6.000 4.200 N Plekho1 n/a
10 TRCN0000166349 CCAGCTCTACATCTCTGAGAA pLKO.1 467 5UTR 100% 4.950 3.465 N PLEKHO1 n/a
11 TRCN0000165452 GATGCTGACCTTGGACTTGAT pLKO.1 915 CDS 100% 4.950 3.465 N PLEKHO1 n/a
12 TRCN0000161670 GTATTTGACCTGAGTGACTAT pLKO.1 519 5UTR 100% 4.950 3.465 N PLEKHO1 n/a
13 TRCN0000165913 GTGAGTCCAGAAGAGAAGGAA pLKO.1 709 5UTR 100% 3.000 2.100 N PLEKHO1 n/a
14 TRCN0000292497 GTGAGTCCAGAAGAGAAGGAA pLKO_005 709 5UTR 100% 3.000 2.100 N PLEKHO1 n/a
15 TRCN0000165209 GTTTACTCTTGCCCACTCCAA pLKO.1 596 5UTR 100% 2.640 1.848 N PLEKHO1 n/a
16 TRCN0000292498 GTTTACTCTTGCCCACTCCAA pLKO_005 596 5UTR 100% 2.640 1.848 N PLEKHO1 n/a
17 TRCN0000163147 GAGAAGGAATCGTGGATCAAT pLKO.1 721 5UTR 100% 5.625 3.375 N PLEKHO1 n/a
18 TRCN0000165914 GTCCAGAAGAGAAGGAATCGT pLKO.1 713 5UTR 100% 3.000 1.800 N PLEKHO1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001420.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03238 pDONR223 100% 57.9% 57.9% None 0_1ins516 n/a
2 ccsbBroad304_03238 pLX_304 0% 57.9% 57.9% V5 0_1ins516 n/a
3 TRCN0000466090 CTCTGTACCGTATTTAGTGGACCC pLX_317 16.1% 57.9% 57.9% V5 0_1ins516 n/a
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