Construct: ORF TRCN0000466090
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012293.1_s317c1
- Derived from:
- ccsbBroadEn_03238
- DNA Barcode:
- CTCTGTACCGTATTTAGTGGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEKHO1 (51177)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466090
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | NM_016274.6 | 100% | 100% | |
| 2 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | XM_024447478.1 | 66.2% | 64.8% | (many diffs) |
| 3 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | NM_001304722.1 | 57.9% | 57.9% | 0_1ins516 |
| 4 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | NM_001304723.1 | 57.9% | 57.9% | 0_1ins516 |
| 5 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | XM_017001420.2 | 57.9% | 57.9% | 0_1ins516 |
| 6 | human | 51177 | PLEKHO1 | pleckstrin homology domain ... | NM_001304724.1 | 55.2% | 55.2% | 0_1ins549 |
| 7 | mouse | 67220 | Plekho1 | pleckstrin homology domain ... | NM_023320.2 | 88% | 90.2% | (many diffs) |
| 8 | mouse | 67220 | Plekho1 | pleckstrin homology domain ... | XM_006501930.2 | 86.3% | 88.8% | (many diffs) |
| 9 | mouse | 67220 | Plekho1 | pleckstrin homology domain ... | XM_011240206.1 | 58.6% | 59.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1293
- ORF length:
- 1227
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gaagaagaac aattccgcca agcggggacc tcaggatgga aaccagcagc 121 ctgcaccgcc cgagaaggtc ggctgggtcc ggaaattctg cgggaaaggg attttcaggg 181 agatttggaa aaaccgctat gtggtgctga aaggggacca gctctacatc tctgagaagg 241 aggtaaaaga tgagaaaaat attcaagagg tatttgacct gagtgactat gagaagtgtg 301 aagagctccg gaagtccaag agcaggagca agaaaaatca tagcaagttt actcttgccc 361 actccaaaca gcccggtaac acggcaccca acctgatctt cctggcagtg agtccagaag 421 agaaggaatc gtggatcaat gccctcaact ctgccatcac ccgagccaag aaccgtatct 481 tggatgaggt caccgttgag gaggacagct atcttgccca tcccactcga gacagggcaa 541 aaatccagca ctcccgccgc cccccaacaa ggggacacct aatggctgtg gcttccacct 601 ctacctcgga tgggatgctg accttggact tgatccaaga ggaagaccct tcccctgagg 661 aaccaacctc ttgtgctgag agctttcggg ttgacctgga caagtctgtg gcccagctgg 721 cagggagccg gcggagagcg gactcagacc gcatccagcc ctccgcagac cgggcaagca 781 gtctctcccg accttgggaa aaaacagaca aaggggccac ctacaccccc caggcaccca 841 agaagttgac gcccacagag aaaggccgct gcgcctccct ggaggagatc ctatctcagc 901 gggatgctgc ctctgcccgc accctccagc tgcgggctga ggaaccccca acccctgccc 961 tccccaaccc ggggcagctg tcccggatcc aggacctggt agcaaggaaa ctggaggaga 1021 ctcaggagct tctggcagag gttcagggac tgggagatgg gaagcgaaag gccaaggacc 1081 cccctcggtc tccgccggat tcTGAGTCAG AGCAGCTGCT GCTGGAGACG GAACGGCTGC 1141 TGGGAGAGGC ATCATCGAAT TGGAGCCAGG CAAAGAGGGT GCTGCAGGAG GTCAGGGAGC 1201 TGAGAGACCT GTACAGACAG ATGGACCTGC AGACCCCGGA CTCCCACCTC AGACAGACCA 1261 CCCCGCACAG TCAGTACCGG AAGAGCCTGA TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1321 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1381 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 1441 CTGTACCGTA TTTAGTGGAC CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1501 ttgtgaaaga tt