Transcript: Human XM_017003222.1

PREDICTED: Homo sapiens RAB, member of RAS oncogene family like 2A (RABL2A), transcript variant X18, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RABL2A (11159)
Length:
2326
CDS:
377..1063

Additional Resources:

NCBI RefSeq record:
XM_017003222.1
NBCI Gene record:
RABL2A (11159)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003222.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419979 TGCATCGTGGTGGCCAATAAA pLKO_005 758 CDS 100% 15.000 7.500 Y RABL2A n/a
2 TRCN0000429189 AGGACTTCATGGATGAGATTT pLKO_005 930 CDS 100% 13.200 6.600 Y RABL2B n/a
3 TRCN0000048164 TCCAAACTCATGGAGAGATTT pLKO.1 479 CDS 100% 13.200 6.600 Y RABL2B n/a
4 TRCN0000424085 AGAGGAGAGCTAGCAGATATG pLKO_005 2169 3UTR 100% 10.800 5.400 Y RABL2A n/a
5 TRCN0000413371 ATGTTGTGAAGCTCTTCAATG pLKO_005 870 CDS 100% 10.800 5.400 Y RABL2A n/a
6 TRCN0000048163 GCCTAGCTATAGTTAGGAATA pLKO.1 1985 3UTR 100% 10.800 5.400 Y RABL2B n/a
7 TRCN0000048112 CGATTAGCTGTGTCTTACAAA pLKO.1 899 CDS 100% 5.625 2.813 Y RABL2A n/a
8 TRCN0000048108 GCCTGCATCATGGTGTTTGAT pLKO.1 662 CDS 100% 5.625 2.813 Y RABL2A n/a
9 TRCN0000048109 CCAGGACTTCATGGATGAGAT pLKO.1 928 CDS 100% 4.950 2.475 Y RABL2A n/a
10 TRCN0000048167 TGATGACAACGTGAAGATCAT pLKO.1 430 CDS 100% 4.950 2.475 Y RABL2B n/a
11 TRCN0000048111 CAAGGGAAGTATGATGCTGAT pLKO.1 413 CDS 100% 4.050 2.025 Y RABL2A n/a
12 TRCN0000048110 GTACGCCCTGACCCTGTACAA pLKO.1 535 CDS 100% 1.650 0.825 Y RABL2A n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1817 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1817 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003222.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02634 pDONR223 100% 98.8% 98.2% None (many diffs) n/a
2 ccsbBroad304_02634 pLX_304 0% 98.8% 98.2% V5 (many diffs) n/a
3 TRCN0000472616 CTCATTTTTGCCGTATCCGTGCAC pLX_317 77% 98.8% 98.2% V5 (many diffs) n/a
4 ccsbBroadEn_11605 pDONR223 100% 97% 96.9% None 408_409insGCA;668_684delinsAA n/a
5 ccsbBroad304_11605 pLX_304 0% 97% 96.9% V5 408_409insGCA;668_684delinsAA n/a
6 TRCN0000468953 ATCGAGGGTCCACGTACCTGTACT pLX_317 56.6% 97% 96.9% V5 408_409insGCA;668_684delinsAA n/a
7 ccsbBroadEn_11606 pDONR223 100% 72% 71% None (many diffs) n/a
8 ccsbBroad304_11606 pLX_304 0% 72% 71% V5 (many diffs) n/a
9 TRCN0000477069 ACGCCCCACAATGCCGACATTTAC pLX_317 65.6% 72% 71% V5 (many diffs) n/a
10 ccsbBroadEn_11604 pDONR223 100% 42.9% 42.9% None (many diffs) n/a
11 ccsbBroad304_11604 pLX_304 0% 42.9% 42.9% V5 (many diffs) n/a
12 TRCN0000471916 TGTCACCTTGGTTTGGGTTGGTTC pLX_317 100% 42.9% 42.9% V5 (many diffs) n/a
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