Transcript: Human XM_017003804.2

PREDICTED: Homo sapiens serine/threonine kinase 36 (STK36), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STK36 (27148)
Length:
4737
CDS:
145..4029

Additional Resources:

NCBI RefSeq record:
XM_017003804.2
NBCI Gene record:
STK36 (27148)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148669 GCCCTGAATCAACATAAACA pXPR_003 GGG 2227 87% 18 0.7422 STK36 STK36 77277
2 BRDN0001145146 TGACATGACCAGCAATAAAG pXPR_003 GGG 758 30% 7 0.6141 STK36 STK36 77276
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017003804.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000272696 GCTGGTCATGTCACCATAATA pLKO_005 907 CDS 100% 15.000 21.000 N STK36 n/a
2 TRCN0000284804 ATGCCATCAGTCCCTGTTATT pLKO_005 4472 3UTR 100% 13.200 18.480 N STK36 n/a
3 TRCN0000380910 GTGACGTTGCTACTCTCTTTA pLKO_005 2498 CDS 100% 13.200 18.480 N STK36 n/a
4 TRCN0000006987 CCGAGATATGAAGCCTCAGAA pLKO.1 513 CDS 100% 4.950 6.930 N STK36 n/a
5 TRCN0000028805 GCATCCCAACATTGTGCATAT pLKO.1 321 CDS 100% 10.800 8.640 N Stk36 n/a
6 TRCN0000006988 CGGGATCTTAGCCTCAGAATT pLKO.1 1209 CDS 100% 0.000 0.000 N STK36 n/a
7 TRCN0000284808 CGGGATCTTAGCCTCAGAATT pLKO_005 1209 CDS 100% 0.000 0.000 N STK36 n/a
8 TRCN0000272648 ATCTTGACTCAGGCCTATAAA pLKO_005 1045 CDS 100% 15.000 10.500 N STK36 n/a
9 TRCN0000381647 ATGGATGCTGACCTCCTTATA pLKO_005 3067 CDS 100% 13.200 9.240 N STK36 n/a
10 TRCN0000284806 TCTGTTGGCTGCATACTATAT pLKO_005 697 CDS 100% 13.200 9.240 N STK36 n/a
11 TRCN0000380142 ATTATCCCTTAGCCAACATTC pLKO_005 4498 3UTR 100% 10.800 7.560 N STK36 n/a
12 TRCN0000006990 CCCAGAACTTCAGGTCCTAAA pLKO.1 978 CDS 100% 10.800 7.560 N STK36 n/a
13 TRCN0000381231 GGAATTTGCAACGAGAGATTG pLKO_005 281 CDS 100% 10.800 7.560 N STK36 n/a
14 TRCN0000006986 GCCAGAGAAGAGTCCTTTCTT pLKO.1 4246 3UTR 100% 5.625 3.938 N STK36 n/a
15 TRCN0000006989 CCAGCATGGAAGTATCCTCAT pLKO.1 2916 CDS 100% 4.050 2.835 N STK36 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017003804.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15041 pDONR223 41.8% 97.8% 19.7% None (many diffs) n/a
2 ccsbBroad304_15041 pLX_304 0% 97.8% 19.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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